流行性感冒病毒鸡胚高产株的遗传特性分析  

Identification of Mutations in a High-yielding Strain of Influenza Virus

作　　者：董婕[1] 张立国[1] 陈爱君[1] 吴昆昱[1] 孙梅生[1] 张智清[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《病毒学报》2002年第4期367-370,共4页Chinese Journal of Virology

基　　金：科研院所技术开发研究专项基金(NCSTE-2000-JKZX-231);"973"计划传染病防治基础研究(973-G19999054108)。

摘　　要：Influenza virus strain A/PR/8/34(H1N1)acquired a high-yielding property after serial passages in fertilized eggsSix internal RNA segments of the high-yielding strain were sequenced from their cloned cDNAs and compared with the corresponding original sequences in the prime A/PR/8/34(H1N1)strainNinety-three point mutations were found accumulated in six genes(PB2,PB1,PA,NP,M,and NS),which lead to 27 amino acid changesA significant low mutation frequency without amino acid change was observed in PA geneThe mutation frequency at the amino acid level was the highest(about 3%)in M2 proteinOur data suggest that the unequal distribution of mutations among different viral proteins may correlate with individual role of each protein in viralInfluenza virus strain A/PR/8/34(H1N1)acquired a high-yielding property after serial passages in fertilized eggsSix internal RNA segments of the high-yielding strain were sequenced from their cloned cDNAs and compared with the corresponding original sequences in the prime A/PR/8/34(H1N1)strainNinety-three point mutations were found accumulated in six genes(PB2,PB1,PA,NP,M,and NS),which lead to 27 amino acid changesA significant low mutation frequency without amino acid change was observed in PA geneThe mutation frequency at the amino acid level was the highest(about 3%)in M2 proteinOur data suggest that the unequal distribution of mutations among different viral proteins may correlate with individual role of each protein in viral growth

关 键 词：流行性感冒病毒 鸡胚高产株 遗传特性分析 高产毒株 基因突变 

分 类 号：R373.13[医药卫生—病原生物学]