狼毒大戟愈伤组织粗多糖的闪式提取工艺优化研究  

Optimization of flash extraction of crude polysaccharides from callus of Euphorbia fischeriana Steud

作　　者：徐亚男 刘懿萱 刘亮亮 王淼 姜军 朴炫春[1] XU Yanan;LIU Yixuan;LIU Liangliang;WANG Miao;JIANG Jun;PIAO Xuanchun(Agricultural College of Yanbian University,Yanji Jilin 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《延边大学农学学报》2022年第4期10-16,共7页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金(81960685)。

摘　　要：狼毒大戟愈伤组织含有较多多糖,为多糖的实际应用提供参考依据,该研究利用闪式提取法进行了多糖的提取。在单因素和响应面设计实验中,通过调控料液比、提取时间和提取温度,对闪式提取工艺进行了优化。结果表明:液料比为40.0∶1(mL∶g),提取时间50 s,提取温度52℃的条件下多糖得率最高,为420.54 mg/g。进而,对狼毒大戟愈伤组织多糖提取物进行了抗氧化能力的测定,发现当提取物浓度为2 mg/mL时,DPPH清除率达到95%。The calluses of Euphorbia fischeriana contain large amounts of polysaccharides.This study used flash extraction method to extract polysaccharides to provide a reference for the practical utilization of the polysaccharides.In single-factor and response surface design experiments,the flash extraction process was optimized by adjusting the liquid-solid ratio,extraction time,and extraction temperature.The results showed that the highest polysaccharide yield(420.5 mg/g)was found at 40∶1(mL∶g)of liquid-solid ratio,50 s of extraction time,and 52℃of extraction temperature.Furthermore,the antioxidant capacity of polysaccharide extract from callus of Euphorbia fischeriana was determined.The DPPH radical scavenging rate reached 95%when the extract concentration was 2 mg/mL.

关 键 词：狼毒大戟 愈伤组织 粗多糖 闪式提取 响应面 

分 类 号：TQ461[化学工程—制药化工]