基质辅助激光解吸电离飞行时间质谱在乳品微生物中的应用进展  

Application Progress of MALDI-TOF MS in Dairy Microbiology

作　　者：金佳佳 严家俊 綦艳 姚丽锋 张娟 黄翠莉 吴思敏 庄艺协 JIN Jiajia;YAN Jiajun;QI Yan;YAO Lifeng;ZHANG Juan;HUANG Cuili;WU Simin;ZHUANG Yixie(Guangdong Testing Institute for Product Quality Supervision,Foshan Guangdong 528300;Technical Center of Gongbei Customs,Zhuhai Guangdong 519015)

机构地区：[1]广东产品质量监督检验研究院,广东佛山528300 [2]拱北海关技术中心,广东珠海519015

出　　处：《中国乳业》2022年第12期89-94,共6页China Dairy

基　　金：国家市场监管总局科技计划项目(2020MK080);广东省质量技术监督局科技项目(2022CZ03);广州市科技计划项目(202102080652);海关总署科研项目(2020HK189);珠海市科技计划项目(ZH22036201210017PWC);广东质检院内部科技项目(2022GQI02)。

摘　　要：微生物污染贯穿乳品工业的各个环节,对经济发展和人类健康构成威胁。对微生物的有效控制是乳品质量与安全重点关注的问题,基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)在乳品安全监管工作中,具有快速、准确、稳定、灵敏以及成本低等特点,因而受到广泛的关注与应用。深入了解MALDITOF MS技术在乳品微生物中的应用途径与策略,可以有效提升乳品安全监管质量与效率。本综述旨在讨论MALDI-TOF MS技术在乳品微生物中的实际应用,对MALDI-TOF MS技术在乳品微生物中的发展趋势和应用前景进行分析和探究,为应用MALDI-TOF MS技术深入研究乳品微生物相关问题提供了理论依据。Microbial contamination runs through all aspects of the dairy industry,being a threat to economic development and human health.Effective control of microorganisms is an important concern of dairy quality and safety.MALDI-TOFMS technology has the characteristics of rapidity,accuracy,stability,sensitivity and low cost in dairy safety supervision,so it has received extensive attention and application.An in-depth understanding of the application approaches and strategies of MALDI-TOFMS technology in dairy microorganisms can effectively increase the quality and efficiency of dairy safety supervision.This review aimed to discuss the practical application of MALDI-TOFMS technique in dairy microbes,the development trend and application prospect of MALDITOFMS technology in dairy microorganisms were analyzed and explored,which provided a theoretical basis for the application of MALDI-TOFMS technology to deeply study dairy microorganism-related issues.

关 键 词：基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS) 食品安全 微生物分析 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]