加味桃红四物汤含量测定、指纹图谱以及抗氧化活性研究  

HPLC Fingerprint and Multi Components Determination in Modified Taohong Siwu Decoction and its Antioxidant Activity

作　　者：张昊祯 杨爱岗 张岩 吴嘉佳 陈跃龙 严诗楷[1] 金慧子[1] ZHANG Hao-zhen;YANG Ai-gang;ZHANG Yan;WU Jia-jia;CHEN Yue-long;YAN Shi-kai;JIN Hui-zi(School of Pharmacy,Shanghai Jiaotong University,Shanghai 200240,China;Shanghai Songhao Biotechnology Company,Shanghai 200331,China)

机构地区：[1]上海交通大学药学院,上海200240 [2]上海松皓生物科技有限公司,上海200331

出　　处：《化学试剂》2023年第1期129-135,共7页Chemical Reagents

摘　　要：建立加味桃红四物汤(白及、桃仁、红花等)HPLC指纹图谱,测定其中没食子酸、苦杏仁苷、绿原酸、芍药内酯苷、羟基红花黄色素A、芍药苷、阿魏酸、洋川芎内酯Ⅰ、毛蕊花糖苷、1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯、苯甲酰芍药苷和藁本内酯共12种成分的含量。色谱柱采用(250 mm×4.6 mm,5μm)Shim-pack VP-ODS柱,柱温30℃;以0.05%磷酸-甲醇为流动相梯度洗脱,体积流量为1.2 mL/min,检测波长分别为210、225、280 nm。10批加味桃红四物汤样品指纹图谱中,共标定26个共有峰,不同批次间相似度均大于0.96,且所有成分在各自范围内线性关系良好(R^(2)≥0.998),平均回收率98.03%~101.43%,RSD为0.72%~1.93%。同时,对加味桃红四物汤的抗氧化活性进行测定,结果显示其清除DPPH、ABTS及羟基自由基的IC_(50)分别为51.11、44.13、275.39μg/mL。该方法准确、简便、快速,可用于抗氧化活性良好的加味桃红四物汤的质量控制。To establish a method for HPLC fingerprints and simultaneous content determination of gallic acid,amygdalin,chlorogenic acid,albiflorin,hydroxysafflor yellow A,paeoniflorin,ferulic acid,senkyunolideⅠ,verbascoside,militarine,benzoylpaeoniflorin and ligustilide in Modified Taohong Siwu Decoction.Shim-pack VP-ODS column(250 mm×4.6 mm,5μm)was used and the column temperature was 30℃.The analysis was performed with methanol-0.05%phosphoric acid,flowing at 1.2 mL/min and the detection wavelengths were set at 210,225,and 280 nm.Among 10 batches of samples,26 common peaks were identified in the HPLC fingerprints with similarities of more than 0.96.Twelve constituents showed good linear relationships(R^(2)≥0.998),whose average recoveries were 98.03%~101.43%with the RSD between 0.72%and 1.93%.The antioxidant activity was evaluated by the scavenging ability of DPPH,ABTS and hydroxyl free radicals.The IC_(50) of DPPH,ABTS and hydroxyl free radicals were 51.11,44.13,and 275.39μg/mL.This rapid,simple and precise method can be used for the quality control of modified Taohong Siwu Decoction with good antioxidant activity.

关 键 词：桃红四物汤 指纹图谱 高效液相色谱 含量测定 抗氧化活性 

分 类 号：R917.101[医药卫生—药物分析学]