气相色谱法测定发酵液菌体中聚羟基脂肪酸酯的样品处理  

Sample treatment for determination of polyhydroxy acid esters in bacteria of fermentation broth by gas chromatography

作　　者：陈月 薛英茹 刘海军 刘安妮 周卫强 杨小凡 李非[2] 安泰 李义 佟毅[3,4] CHEN Yue;XUE Yingru;LIU Haijun;LIU Anni;ZHOU Weiqiang;YANG Xiaofan;LI Fei;AN Tai;LI Yi;TONG Yi(Beijing Key Laboratory of Nutrition,Health and Food Safety,Nutrition&Health Research Institute,COFCO Corporation,Beijing 102209,China;School of Chemical Engineering,Hebei University of Technology,Tianjin 300130,China;COFCO Biochemical Energy(Yushu)Co.,Ltd.,Changchun 161100,China;National Engineering Research Center of Corn Deep Processing,Jilin COFCO Biochemical Co.,Ltd.,Changchun 130033,China)

机构地区：[1]营养健康与食品安全北京市重点实验室,中粮营养健康研究院有限公司,北京100029 [2]河北工业大学化工学院,天津300130 [3]中粮生化能源(榆树)有限公司,长春130401 [4]玉米深加工国家工程研究中心,吉林中粮生化有限公司,长春130033

出　　处：《化学分析计量》2022年第12期6-10,共5页Chemical Analysis And Meterage

基　　金：国家重点研发计划,绿色生物制造专项(2021YFC2101700)。

摘　　要：优化气相色谱检测发酵液菌体中聚羟基脂肪酸酯含量的预处理过程。待测菌体经三氯甲烷及酯化液预处理,通过响应面优化分析方法建立二阶多项式,研究不同预处理参数对发酵液菌体中聚羟基脂肪酸酯检测含量的影响。预处理后的样品采用PH-5色谱柱(30 m×0.32 mm,0.25μm)检测。结果表明,当酯化温度为103℃,酯化时间为3.4 h,发酵液菌体样品质量浓度为12.64 g/L时,可以在较短的酯化时间内完成对菌体的预处理,保证了检测结果准确、可靠。To optimize the pretreatment process for determination of polyhydroxy acid esters in fermentation broth by gas chromatography. The bacteria to be tested were pretreated with trichloromethane and esterification solution, and the second-order polynomial was established by response surface optimization analysis method to study the effects of different pretreatment parameters on the detection content of polyhydroxy acid ester in the bacteria of fermentation solution. The pretreated samples were detected on a PH-5 column(30 m×0.32 mm, 0.25 μm). The results showed that when the esterification temperature was 103 ℃, the esterification time was 3.4 h, and the mass concentration of bacteria samples in fermentation broth was 12.64 g/L, the pretreatment of bacteria could be completed in a short esterification time, and the accuracy of detection could be ensured.

关 键 词：聚羟基脂肪酸酯 响应面优化分析 酯化反应 

分 类 号：TQ316.1[化学工程—高聚物工业]