Nano Calcium-Deficient Hydroxyapatite/O-carboxymethyl Chitosan-CaCl_(2) Microspheres Loadedwith RheinforBone Defect Repair  

作　　者：Zhenzhen Tian Yuanyuan Guo Xiuying Yang Kebing Guo Jingou Ji Shilei Hao 

机构地区：[1]School of Chemistry and Chemical Engineering,Chongqing University,No.55,University City South Road,Shapingba District,Chongqing 401331,China [2]Key Laboratory of Biorheological Science and Technology,Ministry of Education,College of Bioengineering,Chongqing University,Chongqing 400030,China [3]Guizhou KingMed Center for Clinical Laboratory Co.,Ltd,Guizhou 550001,China

出　　处：《Journal of Bionic Engineering》2022年第4期1087-1099,共13页仿生工程学报（英文版）

摘　　要：Glutaraldehyde(GA),the most widely used crosslinking agent for biomaterials,is cytotoxic.CaCl_(2) is of particular interest due to its non-toxic nature.Rhein can chelate Ca^(2+)and promote bone growth.Here we reported a novel nano calcium-deficient hydroxyapatite/O-carboxymethyl chitosan-CaCl_(2) microspheres loaded with rhein(RH-nCDHA/OCMC-CaCl_(2) microspheres)using CaCl_(2) as crosslinking agent for bone defect repair.The obtained microspheres were characterized by X-ray diffraction(XRD),scanning electron microscopy(SEM),thermogravimetric analysis(TG)and Fourier transform infrared spectroscopy(FT-IR).The surface of the obtained microspheres is rough with quite a few voids.The nano calcium-deficient hydroxyapatite(nCDHA)accounts for about 70% of the total weight of the microspheres,which is equivalent to the proportion of inorganic substances in human bones.A high encapsulation efficiency(EE)and loading capacity(LC)of the microspheres loaded with rhein was 90.20±0.60% and 11.03±0.30%,respectively.For microspheres using CaCl_(2) in simulated body fluid(SBF)after 14 days,the drug released continuously and bone-like apatite formed like layer.The cells on the surface of the RH-nCDHA/OCMC-CaCl_(2) microspheres grew better comparing with nCDHA/OCMC-GA microspheres and the skull defects of rats after landfill can be almost repaired after 8 weeks,which revealed the potential of the microspheres for bone repair.

关 键 词：Nano calcium-deficient hydroxyapatite O-carboxymethyl chitosan RHEIN MICROSPHERES Bone repair 

分 类 号：O63[理学—高分子化学]