Effect of electroacupuncture on inflammatory signal expression in local tissues of rats with chronic pelvic pain syndrome based on purinergic 2X7 receptor/NOD-like receptor pyrin domaincontaining 3 signal pathway  

作　　者：XU Chang LI Na WU Xiaoling DAI Xingye YANG Zhiwen SUN Qianhui SHI Tianyu CHAI Yemao PANG Dandan CHENG Kai 

机构地区：[1]Major in Acupuncture and Tuina,school of Acupuncture and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China [2]Experimental science of acupuncture and moxibustion teaching and research section,school of Acupuncture and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China [3]Complex laboratory,school of Acupuncture and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China [4]Meridians and acupoints teaching and research section,school of Acupuncture and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China [5]Academy of Chinese Medical Sciences,Beijing 100700,China

出　　处：《Journal of Traditional Chinese Medicine》2022年第6期965-971,共7页中医杂志（英文版）

基　　金：Supported by Fundamental Research Funds for the Central Universities Project:RNA Sequencing Technology Screening the Mechanism of Acupuncture on Pain in Chronic Pelvic Pain Syndrome Rats (2020-JYB-XJSJJ-018)。

摘　　要：OBJECTIVES: To study the expression of inflammatory signal in local prostate tissue of chronic pelvic pain syndrome(CPPS) rats by electroacupuncture(EA) of Guanyuan(CV4), Zhongji(CV3), Huiyang(BL35) and Sanyinjiao(SP6), and to explore the possible mechanism of anti-inflammatory and analgesic effects of EA. METHODS : A total of 36 Sprague-Dawley male rats were randomly divided into three groups: control, model and EA(n=12 rats/group). The CPPS model was made by injection of CFA into ventral lobes of the prostate(0.1 m L). Electric acupuncture apparatus was applied to stimulate Guanyuan(CV4), Zhongji(CV3), bilateral Huiyang(BL35) and Sanyinjiao(SP6) acupoints in EA group. The general condition of rats was observed and the prostate index(PI) was calculated. The thermal pain threshold was collected after each therapeutic course. Histopathological changes of the prostate tissue were examined by hematoxylin-eosin staining method. The expression levels of tumor necrosis factor α(TNF-α), interleukin-1β(IL-1β) and prostaglandin E2(PGE2) in prostatic homogenates were measured by enzyme linked immunosorbent assay(ELISA). Moreover, the expression levels of purinergic 2X7 receptor(P2X7R), NOD-like receptor pyrin domain-containing 3(NLRP3), caspase-1 and interleukin-18(IL-18) m RNA were quantified by quantitative real-time polymerase chain reaction. RESULTS: Compared with control group, the PI of rats increased, and the thermal pain threshold decreased significantly in model group. The morphological structure of prostate tissues of rats in model group was severely damaged with a large number of inflammatory cells infiltration. Additionally, the levels of TNF-α, IL-1β and PGE2 were higher, and the expressions of P2X7R, NLRP3, caspase-1 and IL-18 m RNA were higher than those in control group. After EA treatment, the PI was significantly decreased, the thermal pain threshold was significantly increased, and the tissue damage was significantly improved. The expressions of inflammatory cytokines were lower in EA group, and ex

关 键 词：ELECTROACUPUNCTURE INFLAMMATORY cytokines receptors purinergic P2X7 NLR family pyrin domain-containing 3 protein signal transduction chronic pelvic pain syndrome 

分 类 号：R245.97[医药卫生—针灸推拿学]