TGFβ1在急性髓系白血病中的异常表达及其对白血病细胞的调控  

Abnormal expression of TGFβ1 in acute myeloid leukemia and its regulation effect on leukemia cells

作　　者：陈文婷 黄莹[1,2] 潘艳萍[1,2] 王述文 唐瑞梅 CHEN Wen-ting;HUANG Ying;PAN Yan-ping;WANG Shu-wen;TANG Rui-mei(Hainan Hospital Affiliated to Hainan Medical University,Haikou 570311,China;Department of Hematology,Hainan Provincial People's Hospital,Haikou 570311,China)

机构地区：[1]海南医学院附属海南医院,海南海口570311 [2]海南省人民医院血液内科,海南海口570311

出　　处：《海南医学院学报》2022年第24期1889-1895,1903,共8页Journal of Hainan Medical University

基　　金：海南省科技厅重点研发项目(ZDYF2019186)。

摘　　要：目的:检测急性髓系白血病(acute myeloid leukemia,AML)患者血液中环氧化酶2(cyclooxygenase-2,COX-2)、转化生长因子β1(transforming growth factor β1,TGFβ1)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达水平,探究TGFβ1对AML细胞增殖、凋亡和周期的作用,为AML的治疗、预后评价提供新的靶点和研究基础。方法:分别收集80名AML患者与60名正常人外周血,使用荧光定量聚合酶链反应和酶联免疫吸附实验分别检测两组外周血单个核细胞和血清中COX-2、TGFβ1、bFGF、VEGF的表达;使用细胞计数试剂盒-8和流式细胞术检测过表达和沉默TGFβ1基因对AML细胞增殖、凋亡和周期的影响。结果:COX-2、TGFβ1、bFGF和VEGF在AML患者外周血单核细胞和血清中的表达量显著上升;TGFβ1促进AML细胞增殖,抑制其凋亡,使G2期细胞比例上升。结论:COX-2、TGFβ1、bFGF、VEGF在AML疾病进展中起到重要作用,TGFβ1可成为AML的诊断及治疗新的潜在靶点。Objective:To explore the level of acute myeloid leukemia(COX-2),transforming growth factor β1(TGFβ1),basic fibroblast growth factor(bFGF),and vascular endothelial growth factor(VEGF)in acute myeloid leukemia(AML)patients and to investigate the role of TGFβ1 on the proliferation,apoptosis and cycle of AML cells,providing new targets and research bases for the treatment and prognostic evaluation of AML. Methods:Peripheral blood was collected from 80 AML patients and 60 normal people. The levels of COX-2,TGFβ1,bFGF and VEGF in peripheral blood mononuclear cells and serum were determined by using quantitative polymerase chain reaction and enzyme linked immunosorbent assay,respectively. The proliferation,apoptosis and cycle of AML cells affected by overexpression and silencing of TGFβ1 was detected using cell counting kit-8 and flow cytometry. Results:The expression of COX-2,TGFβ1,bFGF and VEGF increased significantly in peripheral blood mononuclear cells and serum in AML patients. TGFβ1 promoted AML cell proliferation,inhibited its apoptosis,and increased stage G2 cell proportion. Conclusion:COX-2,TGFβ1,bFGF and VEGF play important roles in the progression of AML. TGFβ1 is a new potential biomarker for the diagnosis and treatment of AML.

关 键 词：急性髓系白血病 诊断靶点 增殖凋亡 TGFΒ1 

分 类 号：R733.71[医药卫生—肿瘤]