二苯甲酮(BP-3)对黄尾蓝魔鱼(Chrysiptera parasema)的生态毒理效应  

Ecotoxicological Effects of Benzophenone-3 Exposure on Liver of Chrysiptera parasema

作　　者：柯怀泱 张彦坤 张纪亮 刁晓平[1,2] Ke Huaiyang;Zhang Yankun;Zhang Jiliang;Diao Xiaoping(Ministry of Education Key Laboratory for Ecology of Tropical Islands,Hainan Normal University,Haikou 571158,China;School of Life Sciences,Hainan Normal University,Haikou 571158,China)

机构地区：[1]热带岛屿生态学教育部重点实验室,海南师范大学,海口571158 [2]海南师范大学生命科学学院,海口571158

出　　处：《生态毒理学报》2022年第5期75-81,共7页Asian Journal of Ecotoxicology

基　　金：海南省重点研发计划项目(ZDYF2021SHFZ253)。

摘　　要：紫外线吸收剂已成为一类新型污染物,对珊瑚礁生态系统具有潜在影响。本研究中,将成年黄尾蓝魔鱼分为溶剂对照组CK(0.1%的乙醇)、1μg·L^(-1)组和10μg·L^(-1)组,于二苯甲酮(benzophenone-3,BP-3)中暴露7 d,取肝脏进行组织学分析,并检测肝脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、脂质过氧化物(MDA)的含量变化,以及抗氧化酶基因的表达,以揭示BP-3对黄尾蓝魔鱼肝脏生态毒理效应。结果表明,暴露7 d后,10μg·L^(-1)组黄尾蓝魔鱼肝细胞肿大,核偏离和细胞核固缩。从抗氧化指标来看,BP-3胁迫后,对肝脏抗氧化酶活性有一定明显的影响。不同浓度BP-3染毒后,SOD酶、CAT酶活性降低,并表现出一定的剂效关系,但与对照组相比没有显著性差异(P>0.05)。MDA含量胁迫后出现先增后降的趋势,低浓度(1μg·L^(-1))BP-3胁迫后,MDA含量显著上升(P<0.01),随着染毒浓度增加含量有所下降。不同基因的表达水平对BP-3胁迫的响应程度有差异,基因bdh2的表达量胁迫以后呈现上升的趋势,SCO1基因的表达量胁迫后出现下降的趋势,均接近显著性水平(P=0.068);CYP27B基因的表达量与对照组相比在1μg·L^(-1)(P<0.05)和10μg·L^(-1)(P<0.01)时显著下降,表现出明显的剂效关系。FTHFD和NADH基因表达量随染毒浓度的上升而变化,但与对照组相比没有差异。综上,MDA含量和CYP27B基因表达水平可以作为敏感的生物标志物来反映BP-3胁迫对黄尾蓝魔鱼的生态毒性效应。本研究揭示了BP-3对黄尾蓝魔鱼肝脏生态毒理效应,为探讨紫外线吸收剂对海洋生态系统的潜在毒性提供了科学依据。UV filters has become one kind of emerging contaminants with potential impacts on coral reef ecosystem.At present study,the toxicological effects of adult Chrysiptera parasema was exposed to benzophenone-3(BP-3),which has been evaluated based on the liver histology and antioxidant defense system.Chrysiptera parasema was exposed to the solvent control groups(CK,0.1%ethanol),1μg·L^(-1)and 10μg·L^(-1)of BP-3 for 7 d.The results showed the hepatocytes of enlarged Chrysiptera parasema,nuclear deviation and nucleus fixation after 7 d exposure at the 10μg·L^(-1)group.Moreover,BP-3 exposure showed significant effects on the antioxidant enzyme activities of the liver.The superoxide dismutase(SOD)and catalase(CAT)activities decreased at different exposure concentrations of BP-3,and there exists an obvious dose-response relationship.However,there was no significant difference between the control group and other treatment groups(P>0.05)for SOD and CAT activities.The malondialdehyde(MDA)content increased significantly(P<0.01)at the concentration of 1μg·L^(-1),and decreased at the concentration of 10μg·L^(-1).The expression levels of different genes were different in response to BP-3 stress,with the expression of bdh2 gene showing an increasing trend and SCO1 gene showing a decreasing trend after BP-3 exposure.The expression of CYP27B gene decreased significantly at 1μg·L^(-1)(P<0.05)and 10μg·L^(-1)(P<0.01)groups compared with the control group,showing a significant dose-response relationship.In conclusion,MDA content and CYP27B gene expression level could be considered as sensitive biomarkers to reflect the ecotoxicological effects of BP-3 on Chrysiptera parasema.This study provides essential information for exploring the potential toxicological effects of BP-type UV filters on marine ecosystems.

关 键 词：二苯甲酮 黄尾蓝魔鱼 肝脏 氧化损伤 

分 类 号：X171.5[环境科学与工程—环境科学]