利用毛细管电泳检测适冷蛋白酶自溶的动态过程  

MONITORING DYNAMIC PROCESS OF PROTEASE AUTOLYSIS WITH CAPILLARY ELECTROPHORESIS

作　　者：孙彩云[1] 陈秀兰[1] 张玉忠[1] 高培基[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《海洋科学》2002年第11期71-72,F003,共3页Marine Sciences

基　　金：国家863计划海洋生物技术;国家自然科学基金;教育部霍英东基金;教育部重点科技计划资助。

摘　　要：Capillary electrophoresis was used to study the autolysis dynamic process of a cold-adapted protease MCP-01, which was produced by a deep-sea cold-adapted bacterium Pseudomonas sp. SM9913. The results showed that cold-adapted protease MCP-01 autolyzed at 20 ℃、30 ℃and 40 ℃. Its autolysis speed became higher and higher with temperature rising. More and more peptide pieces were produced after autolyzing autolysis. Some big peptide pieces produced by protease autolysis still had catalytic activity. Therefore, studying the autolysis dynamic process of a protease with capillary electrophoresis was faster and more convenient than with SDS-PAGE electrophoresis.Capillary electrophoresis was used to study the autolysis dynamic process of a cold-adapted protease MCP-01, which was produced by a deep-sea cold-adapted bacterium Pseudomonas sp. SM9913. The results showed that cold-adapted protease MCP-01 autolyzed at 20 ℃、30 ℃and 40 ℃. Its autolysis speed became higher and higher with temperature rising. More and more peptide pieces were produced after autolyzing autolysis. Some big peptide pieces produced by protease autolysis still had catalytic activity. Therefore, studying the autolysis dynamic process of a protease with capillary electrophoresis was faster and more convenient than with SDS-PAGE electrophoresis.

关 键 词：毛细管电泳 检测 适冷蛋白酶 自溶 动态过程 

分 类 号：Q556.9[生物学—生物化学]