红花石蒜开花过程中不同部位糖类和蛋白质含量变化  

Changes of Carbohydrate and Protein Contents in Different Parts of Lycoris radiata During Flowering

作　　者：张瑶 蔡军火[1] 魏绪英[2] 马美霞 杨玉 吴靖 萨日娜 ZHANG Yao;CAI Junhuo;WEI Xuying;MA Meixia;YANG Yu;WU Jing;SA Rina(College of Landscape and Art,Jiangxi Agricultural University,Nanchang 330045,China;College of Art,Jiangxi University of Finance and Economics,Nanchang 330032,China)

机构地区：[1]江西农业大学园林与艺术学院,江西南昌330045 [2]江西财经大学艺术学院,江西南昌330032

出　　处：《江西农业大学学报》2022年第6期1419-1427,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31960327);江西省自然科学基金项目(20202BABL205004)。

摘　　要：【目的】石蒜属为东亚特有,具有较高的药用和观赏价值。了解石蒜的开花规律,尤其是开花过程中的生理变化,可为有效调控花期提供基础资料。【方法】以不同开花时期红花石蒜的根系、鳞茎、花葶为研究材料,首先采用定株观察法进行开花物候形态学观测,再分别采用蒽酮比色法、考马斯亮蓝法和分光光度法测定了不同器官内的可溶性糖、可溶性蛋白及淀粉、葡萄糖、果糖、蔗糖含量。【结果】(1)在红花石蒜开花过程中,鳞茎内的蔗糖和葡萄糖含量呈逐渐升高趋势,可溶性糖含量则呈先升后降趋势,而淀粉、果糖和可溶性蛋白质的含量变化不显著。(2)在开花过程中,花葶和根系内的6种内含物的含量均有显著变化。其中,从出葶期至始花期,花葶和根系内的可溶性蛋白质和蔗糖含量均呈显著下降趋势,花葶内的葡萄糖含量则持续上升,根系内的葡萄糖含量则呈先升后降趋势;而花葶与根内的果糖含量变化趋势正好相反。(3)不同器官间的6种内含物的含量差异显著。其中,可溶性糖、果糖、蔗糖、可溶性蛋白质均以鳞茎中最高;葡萄糖含量以花葶中最高,而淀粉含量却以根系中最高。(4)鳞茎内葡萄糖的含量变化与开花时期呈极显著正相关,蔗糖的含量变化与开花时期呈极显著负相关;且葡萄糖与可溶性糖、蔗糖的含量变化均呈显著负相关,而与淀粉、果糖含量变化的相关性均不显著。【结论】石蒜开花是一个耗能过程,且以可溶性糖中的蔗糖消耗最为显著。同时,石蒜的开花也是一个能量合成过程,主要表现为体内有大量的葡萄糖积累,这可能与花葶的光合作用有关。[Objective]As a unique plant in East Asia,Lycoris Herb has high medicinal and ornamental value.This study aims to understand the flowering law of Lycoris spp.,especially the physiological changes during flowering,thus providing basic data for effective regulation offlowering period.[Method]The roots and bulbs of Lycoris radiata with different flowering periods were used as the research materials.First,the morphological observation of flowering phenology was conducted by the fixed plant observation method.Then,the contents of soluble sugar,soluble protein,starch,glucose,fructose and sucrose in different organs were determined by anthrone colorimetry,coomassie brilliant blue method and spectrophotometry,respectively.[Results]The research results showed that:(1)During flowering,sucrose and glucose content in bulbs increased gradually,while soluble sugar content increased first and then decreased,while starch,fructose and soluble protein content did not change significantly.(2)During flowering,the contents of six inclusions in scape and root changed significantly.Among them,from the scape stage to the flowering stage,the soluble protein and sucrose content in scape and root showed a significant downward trend,while the glucose content in scape continued to increase,and the glucose content in root showed a trend of increase firstly and then decrease.The variation trend of fructose content in scape and root was opposite.(3)There were significant differences in the contents of six inclusions between different organs.Among them,soluble sugar,fructose,sucrose and soluble protein were the highest in bulbs;glucose content was the highest in scape,while starch content was the highest in root.(4)The change of glucose content in bulbs was significantly positively correlated with flowering period,while the change of sucrose content was in a significantly negative correlation with the flowering period.Moreover,glucose was in a significant negative correlation with the contents of soluble sugar and sucrose,but not significantly c

关 键 词：红花石蒜 开花期 可溶性蛋白 糖类物质 不同器官 

分 类 号：Q949.718.25[生物学—植物学]