钽离子注入对镍钛合金表面生物学性能的影响  

Biological Property Investigation of Nitinol Surface Implanted with Tantalum

作　　者：吴凌 谭继 钱仕[1,3] 葛乃建 刘宣勇 WU Ling;TAN Ji;QIAN Shi;GE Naijian;LIU Xuanyong(State Key Laboratory of High Performance Ceramics and Superfine Microstructure,Shanghai Institute of Ceramics,Chinese Academy of Sciences,Shanghai 200050,China;Center of Materials Science and Optoelectronics Engineering,University of Chinese Academy of Science,Beijing 100049,China;Cixi Center of Biomaterials Surface Engineering,Ningbo 315300,China;Eastern Hepatobilialy Surgery Hospital,Naval Medical University,Shanghai 200438,China)

机构地区：[1]中国科学院上海硅酸盐研究所,高性能陶瓷与超微结构国家重点实验室,上海200050 [2]中国科学院大学材料科学与光电工程中心,北京100049 [3]慈溪生物材料表面工程中心,宁波315300 [4]海军军医大学东方肝胆外科医院,上海200438

出　　处：《无机材料学报》2022年第11期1217-1224,共8页Journal of Inorganic Materials

基　　金：国家自然科学基金面上项目(31971249);上海市基础研究重点项目(19JC1415500);宁波市高层次人才和高端创新创业团队引进计划(2018A-09-C)。

摘　　要：镍钛合金血管支架植入后可引发血栓和支架再狭窄,且对损伤的血管内壁无修复作用,需进行表面改性赋予其抗凝血和促内皮化生物学功能。本研究采用等离子体浸没离子注入与沉积(PIII&D)技术将钽(Ta)注入至镍钛合金,研究Ta离子注入对镍钛表面理化特性及生物学性能的影响规律。结果表明,调控Ta离子注入时间,可在镍钛表面分别构建含Ta、Ta/Ta_(2)O_(5)、Ta/Ta_(2)O_(5-x)/Ta_(2)O_(5)三种不同组分的改性层。各种改性样品中,含Ta/Ta_(2)O_(5-x)/Ta_(2)O_(5)的改性镍钛表面亲水性均更好,可提供更多细胞附着位点,促进人脐静脉内皮细胞早期粘附和铺展,并提高其增殖能力。相比仅含单质Ta的改性镍钛表面,含Ta/Ta_(2)O_(5-x)/Ta_(2)O_(5)改性镍钛表面的血液相容性更高,血小板粘附数量显著减少,且基本保持为未被激活的球形状态;各组改性表面的溶血率远低于5%阈值,均未发生明显溶血现象。上述结果说明,Ta离子注入改性镍钛血管支架在降低血栓形成、加速内皮化方面具有潜在应用。Nitinol vascular stent can trigger thrombosis and restenosis,but lacks the ability to rehabilitate with injury still to the blood vessel wall.Thus,surface modification to the stent is indispensable for nitinol surfaces to realize biological functions of anticoagulation and accelerate endothelialization.Here,tantalum ions were implanted into nitinol surfaces by plasma immersion ion implantation and deposition(PIII&D).Physicochemical and biological characteristics of the modified nitinol surfaces were analyzed.The results show that the Ta,Ta/Ta_(2)O_(5),and Ta/Ta_(2)O_(5-x)/Ta_(2)O_(5)-containing modified layers are formed on nitinol surfaces with extension of implantation time.Among all samples,the modified surface containing Ta/Ta_(2)O_(5-x)/Ta_(2)O_(5) phase possesses better hydrophilicity and provides more sites for cell attachment to promote initial cell adhesion and proliferation.Compared with the solely Ta-bearing surface,the modified surface containing Ta/Ta_(2)O_(5-x)/Ta_(2)O_(5) shows superior hemocompatibility,on which fewer adhered platelets are observed,maitaining an inactive and original spherical shape.Besides,the hemolysis rates of all samples are far below the threshold value of 5%,indicating that hemolysis hardly happens.The results indicate that Ta ion implantation modified nitinol stent has a potential application in reducing thrombosis and accelerating endothelialization.

关 键 词：镍钛合金 钽 离子注入 血液相容性 

分 类 号：TQ174[化学工程—陶瓷工业]