Bioinspired Design of Reversible Fluorescent Probes for Tracking Nitric Oxide Dynamics in Live Cells  

作　　者：Rui-Ying Guo Yu-Tian Zhang Supphachok Chanmungkalakul Hao-Ran Guo Yongzhou Hu Jia Li Xiaogang Liu Yi Zang Xin Li 

机构地区：[1]College of Pharmaceutical Sciences,Zhejiang University,Hangzhou 310058 [2]State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203 [3]University of Chinese Academy of Sciences,Beijing 100049 [4]Fluorescence Research Group,Singapore University of Technology and Design,Singapore 487372 [5]Open Studio for Druggability Research of Marine Natural Products,Pilot National Laboratory for Marine Science and Technology,Qingdao 266237

出　　处：《CCS Chemistry》2021年第10期116-128,共13页中国化学会会刊（英文）

基　　金：supported by the National Natural Science Foundations of China(nos.22077112,21778048,81673489,31871414,and 81125023);Natural Science Foundation of Zhejiang Province,China(no.LR18H300001);National Science and Technology Major Project“Key New Drug Creation and Manufacturing Program”(nos.2018ZX09711002-010-004,2018ZX09711002-007-002,2019ZX09201001-003-009,2019ZX09201001-003-010,and 2019ZX09201001-004-010),K.C.Wong Education Foundation,and Singapore University of Technology and Design(SUTD)[SUTD-ZJU IDEA grant nos.T1SRCI17126 and SUTD-ZJU(VP)201905].

摘　　要：Nitric oxide(NO)participates in various pathways and revealing its dynamics is critical for resolving its pathophysiology.While there are methods available for detecting biological NO,few are capable of tracking NO dynamics.Herein,inspired by the cellular machinery of reversible thiol modification by NO,we have successfully designed a family of cysteine analogues tagged with fluorophores for visualizing cellular NO dynamics.

关 键 词：fluorescent probe fluore scence imaging REVERSIBLE nitric oxide S-NITROSATION 

分 类 号：O62[理学—有机化学]