苯扎贝特联合维甲酸对APPswe M17细胞分化的影响  

Synergistic effect of bezafibrate and retinoic acid on differentiation of APPswe M17 cells

作　　者：王祯莲 宋国强[2] 祝雄伟 WANG Zhenlian;SONG Guoqiang;ZHU Xiongwei(College of Chemistry&Materials Engineering,Wenzhou University,Wenzhou 325035,China;School of Pharmacy,Changzhou University,Changzhou 213164,China;Department of Pathology,Case Western Reserve University,Cleveland 44106,USA)

机构地区：[1]温州大学化学与材料工程学院,浙江温州325035 [2]常州大学药学院,江苏常州213164 [3]凯斯西方储备大学病理系,俄亥俄州克利夫兰44106

出　　处：《常州大学学报（自然科学版）》2023年第1期86-92,共7页Journal of Changzhou University：Natural Science Edition

基　　金：国家自然科学基金青年基金资助项目(81201657)。

摘　　要：通过Western blot法、细胞形态检测等方法研究联合运用苯扎贝特(Bezafibrate,Beza)和维甲酸(Retinoic acid,RA)诱导Appswe M17细胞向神经元样细胞分化的作用和机制。结果发现,Vector M17细胞几乎不表达APP,APPswe M17细胞表达高水平的APP。10μmol/L RA可以诱导Vector M17分化为神经元样细胞,联合运用250μmol/L Beza和10μmol/L RA可以诱导APPswe M17细胞分化为神经元样细胞,其作用机制是通过上调APPswe M17细胞中的DLP1的表达恢复APPswe M17细胞的分化潜能。The paper studied the effect and mechanism of combined use of bezafibrate(Beza)and retinoic acid(RA)to induce the differentiation of APPswe M17 cells into neuron like cells by Western blot and cell morphology detection.The results showed that vector M17 cells hardly expressed APP,while APPswe M17 cell line expressed high levels of APP.10μmol/L RA could induce vector M17 to differentiate into neuron like cells,250μmol/L Beza and 10μmol/L RA could induce the differentiation of APPswe M17 cells into neuron like cells.Its mechanism is to restore the differentiation potential of APPswe M17 cells by up-regulating the expression of DLP1 in APPswe M17 cells.

关 键 词：苯扎贝特 全反式维甲酸 APPswe M17细胞 细胞分化 

分 类 号：R741.02[医药卫生—神经病学与精神病学]