马铃薯bZIP家族的鉴定与表达分析  

Identification and expression analysis of bZIP family in potato

作　　者：李想 冯建英 鲁黎明[1] 李立芹[1] LI Xiang;FENG Jian-ying;LU Li-ming;LI Li-qin(College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学农学院,四川成都611130

出　　处：《江苏农业学报》2022年第6期1453-1464,共12页Jiangsu Journal of Agricultural Sciences

基　　金：四川省科技厅项目(2018JY0078)。

摘　　要：为探索马铃薯中bZIP的功能,对马铃薯bZIP基因家族进行鉴定,分析其系统进化关系、染色体定位、植物组织表达模式,并对非生物逆境胁迫条件下的转录组数据进行分析。利用荧光定量PCR技术检测12个bZIP基因在马铃薯根、茎、叶以及在干旱、低钾、高盐、脱落酸(ABA)和H2O2等胁迫下的表达情况。通过对马铃薯bZIP转录因子进行全基因组检索,得到104个bZIP基因家族成员。其编码的bZIP转录因子保守结构域长度为21~79 aa,相对分子质量为13 088.03~88 180.52,等电点为5.02~9.96,均属于亲水蛋白质。利用在线软件MEME对bZIP家族成员的蛋白质全长氨基酸序列进行结构分析,并根据其结构进行相似性聚类,共得到20个Motif,其中Motif1存在于104个bZIP中,为bZIP家族的特征基序。根据进化树分析结果,将马铃薯bZIP家族分为10个亚族。基于转录组数据的表达模式分析结果表明,马铃薯bZIP成员广泛参与ABA、盐、干旱等非生物胁迫响应。qPCR分析结果显示,马铃薯bZIP家族成员还能够在低钾、H2O2胁迫应答中发挥作用。综合分析可知,马铃薯bZIP家族基因广泛参与了非生物胁迫响应,研究结果为马铃薯bZIP家族的功能研究奠定了基础。To explore the function of bZIP in potato, the bZIP gene family in potato was identified, and its phylogenetic relationship, chromosome mapping, plant tissue expression pattern and transcriptome data under abiotic stress conditions were analyzed. Fluorescence quantitative PCR was used to detect the expression of 12 bZIP genes in roots, stems, leaves and under drought, low potassium, high salt, abscisic acid(ABA) and H2O2stress. A total of 104 bZIP gene family members were obtained by genome-wide retrieval of potato bZIP transcription factors. The length of the conserved domain ranged from 21 aa to 79 aa, the relative molecular weight ranged from 13 088.03 to 88 180.52, and the isoelectric point ranged from 5.02 to 9.96. They were all hydrophilic proteins. The structure of the full-length amino acid sequence of bZIP family members was analyzed by MEME, and the similarity clustering was performed according to its structure. A total of 20 motifs were obtained. Motif1 was found in 104 bZIPs and was the characteristic motif of the bZIP family. According to the results of phylogenetic tree analysis, the potato bZIP family was divided into ten subgroups. Analysis of expression patterns based on transcriptome data indicated that potato bZIP members were extensively involved in abiotic stress responses such as ABA, salt, and drought. The results of qPCR analysis showed that bZIP family members could also play a role in response to low potassium and H2O2stress. Genes of the potato bZIP family are widely involved in the response to abiotic stress, and the results of this study can lay a foundation for the functional study of the potato bZIP family.

关 键 词：马铃薯 bZIP基因 表达模式 非生物胁迫 

分 类 号：S532.01[农业科学—作物学]