药用植物罗汉果活性成分的抗运动性疲劳作用  

Anti-exercise Fatigue Effect of Active Components of Medicinal Plant Siraitia grosvenorii(Swingle)

作　　者：高文强[1] 温织琳[1] Gao Wenqiang;Wen Zhilin(Guilin University of Electronic Technology,Guilin,541004)

机构地区：[1]桂林电子科技大学,桂林541004

出　　处：《分子植物育种》2022年第23期7923-7930,共8页Molecular Plant Breeding

基　　金：广西高等教育本科教学改革工程项目(2016JGB215)资助。

摘　　要：罗汉果[Siraitia grosvenorii (Swingle)]是一种主要产于广西壮族自治区的多年生草本藤本植物,罗汉果醇(mogrol, MO)是罗汉果甜苷的生物代谢物。为了探讨药用植物罗汉果活性成分罗汉果醇(mogrol, MO)的抗运动性疲劳作用及机制,本研究通过负重力竭游泳实验建立疲劳大鼠模型,使用相应的试剂盒检测肝糖原和肌糖原含量、血清指标水平和骨骼肌中抗氧化指标水平。通过Western blot检测骨骼肌中磷酸化-AMP依赖的蛋白激酶α (p-AMPKα)、AMPKα、过氧化物酶体增殖物激活受体γ (PPARγ)共激活因子α (PGC-1α)和线粒体转录因子A (TFAM)的蛋白表达水平。结果显示,罗汉果醇延长了疲劳大鼠的力竭游泳时间(P<0.05),升高了肝糖原和肌糖原含量(P<0.05),降低了血清乳酸(LA)、尿素氮(BUN)、肌酸激酶(CK)、谷丙转氨酶(ALT)和谷草转氨酶(AST)水平(P<0.05)。罗汉果醇升高了疲劳大鼠骨骼肌中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)水平,降低了丙二醛(MDA)水平(P<0.05)。罗汉果醇升高了疲劳大鼠骨骼肌中p-AMPKα/AMPKα、PGC-1α和TFAM的蛋白相对表达量(P<0.05)。本研究表明罗汉果醇可能通过增加糖原储备、提高抗氧化活性、激活AMPK信号通路发挥抗疲劳作用。Siraitia grosvenorii(Swingle) is a perennial herb vine mainly produced in Guangxi Zhuang Autonomous Region, and mogrol(MO) is the biological metabolite of mogroside. In order to explore the anti-exercise fatigue effect and mechanism of mogrol(MO), an active component of medicinal plant Siraitia grosvenorii, this study established a fatigue rat model by weight bearing exhaustive swimming test, and used the corresponding kit to detect the content of liver glycogen and muscle glycogen, serum index level and the level of antioxidant index in skeletal muscle. Detection of phosphorylated AMP dependent protein kinase in skeletal muscle by Western blot α(p-AMPKα), AMPKα, peroxisome proliferator activated receptor γ(PPARγ), coactivator α(PGC-1α) and mitochondrial transcription factor A(TFAM). The results showed that siranol increased the exhausted swimming time of fatigue rats(P<0.05), increased the content of liver glycogen and muscle glycogen(P<0.05), and decreased the levels of serum lactic acid(LA), urea nitrogen(BUN), creatine kinase(CK), alanine aminotransferase(ALT), and aspartate aminotransferase(AST)(P<0.05). Siranol increased the levels of superoxide dismutase(SOD) and catalase(CAT) in skeletal muscle of fatigue rats, and decreased the level of malondialdehyde(MDA)(P<0.05). Siranol increases p-AMPK in skeletal muscle of fatigue rats α/AMPKα, PGC-1α and TFAM(P<0.05). This study suggests that siranol may play an anti-fatigue role by increasing glycogen reserves, enhancing antioxidant activity and activating AMPK signaling pathway.

关 键 词：罗汉果 罗汉果醇 抗疲劳 糖原 抗氧化活性 AMPK信号通路 

分 类 号：R285.5[医药卫生—中药学]