SH-6联合LY294002对胶质瘤细胞增殖、凋亡的影响及机制探究  

The effect of SH-6 combined with LY294002 on the proliferation and apoptosis of glioma cells and its mechanism

作　　者：邹有瑞[1] 李嘉麟 黄灵 马悦 马辉[1] ZOU Yourui;LI Jialin;HUANG Lin;MA Yue;MA Hui(Department of Neurosurgery,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院神经外科,宁夏银川750004 [2]宁夏医科大学,宁夏银川750004

出　　处：《宁夏医学杂志》2022年第12期1057-1060,共4页Ningxia Medical Journal

基　　金：国家自然科学基金项目(81760449)。

摘　　要：目的 探究磷脂酰肌醇磷酸类似物SH-6联合PI3K/AKT信号通路抑制剂LY294002对胶质瘤细胞增殖、凋亡的影响及作用机制。方法 使用SH-6及LY294002处理胶质瘤细胞,并设置分组,分为对照组、DMSO组、联合组,使用Western-Blot检测p-PI3K、PI3K、p-AKT、AKT蛋白的表达水平。分别运用流式细胞实验、CCK-8实验、划痕实验、Transwell等方法观察在不同实验条件下胶质瘤细胞周期、增殖、凋亡、侵袭及转移功能变化情况。结果 Western-Blot检测到联合组的胶质瘤细胞中p-PI3K、p-AKT蛋白的表达量均降低(P<0.05),PI3K、AKT总蛋白的表达量无明显变化。联合组处于G0/G1期的胶质瘤细胞比例明显高于对照组、DMSO组(P<0.05),胶质瘤细胞增殖、侵袭和转移能力明显减弱,凋亡率显著增加(P<0.05)。结论 SH-6联合LY294002能够调节PI3K/AKT信号通路中PI3K和AKT蛋白修饰的磷酸化,进而影响胶质瘤细胞增殖、凋亡、侵袭和转移等生物学功能。Objective To explore the effect of SH-6 combined with LY294002 on the proliferation and apoptosis of glioma cells and its mechanism.Methods SH-6 and LY294002 were used to treat glioma cells, and they were divided into control group, DMSO group and combined group(combined). Western-Blot was used to detect the expression levels of p-PI3K,PI3K,p-AKT,and AKT proteins. The changes of glioma cell cycle, proliferation, apoptosis, invasion and metastasis were observed by using flow cytometry experiment, CCK-8 experiment, scratch experiment, Transwell and other methods.Results Western Blot detection of p-PI3K and p-AKT expression in glioma cells in the combined group was decreased, and the expression of PI3K and AKT did not change significantly. The proportion of cells in the G0/G1 phase of the cell cycle in the combined group was significantly higher than that in the control group and DMSO group(P<0.05). The proliferation, invasion and metastasis ability of glioma cells were significantly weakened, and the apoptosis rate was significantly increased(P<0.05).Conclusion SH-6 combined with LY294002 can regulate the phosphorylation of proteins in the PI3K/AKT signaling pathway, thereby affecting the biological functions of glioma cells such as proliferation, apoptosis, invasion and metastasis.

关 键 词：胶质瘤 SH-6 LY294002 侵袭 PI3K AKT 

分 类 号：R739.41[医药卫生—肿瘤]