厌氧微生物菌群XH-1对2,4,6-三氯苯酚的降解特性  

Degradation characteristics of 2,4,6-trichlorophenol by the anaerobic consortium XH-1

作　　者：孙百惠 宋雪英[1] 严俊 杨毅 吕燕 王晶晶 宋玉芳[2] 李秀颖[2] SUN Bai-hui;SONG Xue-ying;YAN Jun;YANG Yi;LYU Yan;WANG Jing-jing;SONG Yu-fang;LI Xiu-ying(Key Laboratory of Ecological Restoration of Regional Polluted Environment,Ministry of Education,Shenyang University,Shenyang 110044,China;Key Laboratory of Pollution Ecology and Environmental Engineering,Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;Gansu Institute of Science and Technology Information,Lanzhou 730099,China)

机构地区：[1]沈阳大学区域污染环境生态修复教育部重点实验室,沈阳110044 [2]中国科学院沈阳应用生态研究所污染生态与环境工程重点实验室,沈阳110016 [3]甘肃省科学技术情报研究所,兰州730099

出　　处：《应用生态学报》2022年第12期3395-3402,共8页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(41907220);环境化学与生态毒理学国家重点实验室开放基金项目(KF2017-08);沈阳市中青年人才科技支持计划项目(RC190371)资助。

摘　　要：有机污染物2,4,6-三氯苯酚(2,4,6-TCP)普遍存在于地下水和河流底泥等厌氧环境中。为了探究厌氧微生物菌群XH-1对2,4,6-TCP的降解能力,本研究以2,4,6-TCP为底物,接种XH-1建立微宇宙培养体系,并以中间产物4-氯苯酚(4-CP)和苯酚为底物分别进行分段富集培养,利用高效液相色谱分析底物的降解转化,同时基于16S rRNA基因高通量测序分析微生物群落结构变化。结果表明:2,4,6-TCP(122μmol·L^(-1))以0.15μmol·d^(-1)的速率在80 d内被完全降解转化,降解中间产物分别为2,4-二氯苯酚(2,4-DCP)、4-氯苯酚和苯酚,所有中间产物最终在325 d被完全降解。高通量测序结果表明,脱卤杆菌和脱卤球菌可能驱动2,4,6-TCP还原脱氯,其中,脱卤球菌可能在4-CP的脱氯转化中发挥重要作用,并与丁酸互营菌和产甲烷菌联合作用彻底降解2,4,6-TCP。Organic pollutant 2,4,6-trichlorophenol(2,4,6-TCP) is commonly found in anaerobic environments such as sediments and groundwater aquifers. To investigate the ability of the anaerobic consortium XH-1 to degrade 2,4,6-TCP, we established anaerobic incubations using 2,4,6-TCP as the substrate and inoculated the incubations with XH-1. Additional subcultures were established by amending with intermediate product 4-chlorophenol(4-CP) or phenol as the substrate. The transformation products of 2,4,6-TCP were analyzed and determined using high-performance liquid chromatography(HPLC). Microbial community structure and key microbial groups involved in the degradation of 2,4,6-TCP were analyzed based on 16 S rRNA gene high-throughput sequencing. The results showed that the initial 122 μmol·L^(-1)2,4,6-TCP was completely transformed after a 80-day incubation at a rate of 0.15 μmol·d-1. 2,4-dichlorophenol(2,4-DCP), 4-CP and phenol were identified as the intermediate products. All intermediate products generated from 2,4,6-TCP transformation were completely degraded after being incubated for 325 days. The main microbial groups responsible for the reductive dechlorination of 2,4,6-TCP might be the organohalide respiring Dehalobacter and Dehalococcoides. The subsequent reductive dechlorination of 4-CP to phenol was likely driven by Dehalococcoides. The cooperation between the organohalide respiring bacteria, Syntrophorhabdus and methanogens(e.g. Methanosaeta and Methanofolis) was responsible for the complete degradation of 2,4,6-TCP.

关 键 词：氯代苯酚 厌氧生物降解 富集培养 还原脱氯 

分 类 号：X172[环境科学与工程—环境科学]