异黄酮类化合物抗奥密克戎毒株作用的研究  

A Preliminary Study onEffect of Isoflavones Against Omicron Strain

作　　者：常红 许寅聪 段绪红 张丹 孙永[4] 袁媛[4] 刘建芳 王鑫 王绛辉 苏彦雷 孙殿兴 CHANG Hong;XU Yincong;DUAN Xuhong;ZHANG Dan;SUN Yong;YUAN Yuan;LIU Jianfang;WANG Xin;WANG Jianghui;SU Yanlei;SUN Dianxing(Hebei Province Academy of Chinese Medicine Sciences,Shijiazhuang 050030,Hebei,China;The First Hospital of Hebei Medical University,Shijiazhuang 050031,Hebei,China;School of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,Hebei,China;Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,Anhui,China;Traditional Chinese Medicine Hospital of Hebei Provincial,Shijiazhuang 050011,Hebei,China;Hebei Eye Hospital,Xingtai 054001,Hebei,China;The 980th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Shijiazhuang 050082,Hebei,China)

机构地区：[1]河北省中医药科学院,河北石家庄050030 [2]河北医科大学第一医院,河北石家庄050031 [3]河北中医学院药学院,河北石家庄050200 [4]安徽省疾病预防控制中心,安徽合肥230601 [5]河北省中医院,河北石家庄050011 [6]河北省眼科医院,河北邢台054001 [7]联勤保障部队第九八〇医院,河北石家庄050082

出　　处：《中华中医药学刊》2022年第12期20-22,I0028,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81672041);河北省自然科学基金(H202106198);河北省卫生健康委员会科研基金(20191055);河北省中医药管理局科研计划(2016019)。

摘　　要：目的探讨异黄酮类化合物抗新型冠状病毒的活性及作用机制。方法利用Autodock Vina软件将毛蕊异黄酮、毛蕊异黄酮苷、染料木素、染料木苷对接到新型冠状病毒RNA聚合酶晶体结构7VB2的底物结合位点处,探讨目标化合物与靶酶活性位点间的相互作用,再利用奥密克戎毒株转染的Vero细胞模型测试其抗病毒活性。结果毛蕊异黄酮、毛蕊异黄酮苷、染料木素、染料木苷均可结合于靶酶7VB2的底物进入通道处而阻碍正常核苷酸类底物的进入,对靶酶执行的核酸复制生物学功能起到抑制作用;初步细胞水平抗病毒活性测试实验仅发现毛蕊异黄酮和染料木素具有抑制奥密克戎毒株核酸复制的作用。结论毛蕊异黄酮和染料木素具有一定抗新型冠状病毒及其变异株的药理活性,实验数据可为抗新型冠状病毒药物开发提供一定的参考。Objective To study the activity and mechanism of action of isoflavones against SARS-CoV-2.Methods The Autodock Vina software was used to dock calycosin,calycosin-7-glucoside,genistein and genistin to the substrate binding site of SARS-CoV-2 RNA polymerase crystal structure 7 VB2 to study the interaction between the target compounds and the active site of the target enzyme.The antiviral activity of these compound monomers was then tested by using the Vero cell model transfected with the Omicron strain.Results Calycosin,calycosin-7-glucoside,genistein and genistin could all bind to the substrates of the target enzyme 7 VB2 to enter the channel and blocked the entry of normal nucleotide substrates,thus inhibiting the biological function of nucleic acid replication performed by the target enzyme.The preliminary antiviral activity tests at the cellular level only revealed that calycosin and genistein inhibited nucleic acid replication of the Omicron strain.Conclusion Calycosin and genistein have some certain pharmacological activities against SARS-CoV-2 and its variants,and the experimental data may provide a certain reference for the development of anti-SARS-CoV-2 drugs.

关 键 词：奥密克戎毒株 RNA聚合酶 异黄酮 分子对接 活性测试 

分 类 号：R259.631[医药卫生—中西医结合]