岩黄连总生物碱提取工艺及抗氧化活性研究  

Study on Extraction Technology and Antioxidant Activity of Total Alkaloids from Corydalis saxicola Bunting

作　　者：李伶 唐文迪 陈寿涣 宁裕龙 覃江克 LI Ling;TANG Wendi;CHEN Shouhuan;NING Yulong;QIN Jiangke(School of Chemistry and Pharmaceutical Sciences,Guangxi Normal University,Guilin Guangxi 541004,China;State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources(Guangxi Normal University),Guilin Guangxi 541004,China)

机构地区：[1]广西师范大学化学与药学学院,广西桂林541004 [2]省部共建药用资源化学与药物分子工程国家重点实验室(广西师范大学),广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2022年第6期145-153,共9页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(21462007);广西创新驱动发展项目(桂科AA18118015-4)。

摘　　要：以脱氢卡维丁得率为评价指标,采用单因素实验及正交试验确定岩黄连中总生物碱的最佳提取工艺,并采用自由基清除法评价其抗氧化活性。实验结果表明,岩黄连总生物碱的最优提取工艺为:提取温度80℃,提取时间2.5 h,乙醇体积分数60%,料液比1∶14,提取3次,在此条件下得率为7.34%;抗氧化活性实验表明,岩黄连生物碱粗提物对DPPH自由基、超氧阴离子自由基有较好的清除作用,对ABTS自由基和羟基自由基有一定程度的清除作用,呈现浓度依赖性,表明岩黄连生物碱粗提物是潜在的天然抗氧化剂。The extraction process of total alkaloids from Corydalis saxicola Bunting was studied by single factor test and orthogonal test,using the yield of dehydrocavidine as the evaluation index.The antioxidant activity of total alkaloids of C.saxicola Bunting was evaluated by free radical scavenging method in vitro.The results showed that the optimal extraction conditions of total alkaloids were as follows:extraction temperature 80℃,extraction time 2.5 h,ethanol concentration 60%,solid-liquid ratio 1∶14,extraction for 3 times,under this condition the total alkaloids yield of C.saxicola Bunting was 7.34%.The antioxidant experiments showed that the crude extract of total alkaloids from C.saxicola Bunting had good scavenging effect on DPPH·and superoxide radical,and had a certain scavenging effect on ABTS+·and hydroxyl radical,showing in a concentration dependent manner,whick revealed that the crude extract of total alkaloids from C.saxicola Bunting are potential natural antioxidants.

关 键 词：岩黄连 生物碱 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]