高温胁迫下景宁木兰淀粉与蔗糖代谢途径转录分析  

Transcriptional analysis of starch and sucrose metabolism pathways in Magnolia sinostellata under heat stress

作　　者：郑晨璐 王倩颖[2] 陆丹迎 申亚梅[2] 马晶晶 刘璐[2] 王云 ZHENG Chenlu;WANG Qianying;LU Danying;SHEN Yamei;MA Jingjing;LIU Lu;WANG Yun(School of Design,Shanghai Jiao Tong University,Shanghai 200240,China;College of Landscape and Architecture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]上海交通大学设计学院,上海200240 [2]浙江农林大学风景园林与建筑学院,浙江杭州311300

出　　处：《浙江农林大学学报》2023年第1期55-63,共9页Journal of Zhejiang A&F University

基　　金：浙江省农业新品种选育重大科技专项(2021C2071-3)。

摘　　要：【目的】从分子生物学角度探究景宁木兰Magnolia sinostellata能否适应城市高温环境,为木兰属Magnolia植物城市推广应用和胁迫分子研究奠定基础。【方法】采取人工控制实验,对景宁木兰幼苗进行40℃极端高温处理,测定果糖、葡萄糖、蔗糖、淀粉碳同化产物,以及果糖磷酸酶、蔗糖磷酸合成酶,进行了转录组测序。【结果】随着胁迫时间的延长,景宁木兰叶片果糖、葡萄糖、蔗糖、淀粉质量分数发生一定的变化,但是差异不显著(P>0.05),果糖合成酶活性呈现显著下降趋势(P<0.05),蔗糖合成酶变化不显著(P>0.05)。转录组数据进一步揭示了在高温胁迫下,相比于24 h,48 h时景宁木兰叶片调节淀粉合成的SS(Unigene 40295)、Glc-1-pa(Unigene 38453)、GBE(CL4668.contig3)基因的表达量增加,随着高温胁迫的加深,调节蔗糖合成的SPS基因呈现下降趋势,并通过荧光定量验证了以上结果。【结论】景宁木兰对极端高温(40℃)有一定的短时耐受性,为应对高温胁迫,不但碳同化产物发生显著变化,调控淀粉与蔗糖代谢途径的关键基因表达也发生了变化,进一步证明了高温会导致景宁木兰叶片内蔗糖和淀粉的相互转化。[Objective]The purpose is to explore whether the shrub species of Magnolia sinostellata could adapt to the high temperature in the city and promote its application.[Method]The 1-year-old seedlings of M.sinostellata were treated with high temperature at 40℃to determine the fructose,glucose,sucrose,starch carbon assimilates,fructose phosphatase,sucrose phosphate synthase,and transcriptome sequencing.[Result]With the extension of stress time,the contents of fructose,glucose,sucrose and starch in leaves of M.sinostellata were changed,but the difference was not significant(P>0.05).The fructose synthase was significantly decreased(P<0.05),but the sucrose synthase was not significantly changed(P>0.05).Transcriptomic data further revealed that SS(Unigene 40295),Glc-1-pa(Unigene 38453)and GBE(CL4668.contig3)regulated starch synthesis at 48 h compared with 24 h under heat stress.The expression of GBE(CL4668.contig3)gene increased,and the SPS gene that regulates sucrose synthesis decreased with the deepening of heat stress.The above results were verified by qPCR.[Conclusion]All results indicated that M.sinostellata had a certain short-term tolerance to extreme temperature of 40℃.In order to cope with high temperature stress,not only the carbon assimilates were significantly changed,but also the expression of key genes regulating starch and sucrose metabolism were changed.It was further proved that high temperature could lead to mutual conversion of sucrose and starch in the leaves of M.sinostellata.

关 键 词：高温 碳同化产物 代谢途径 关键基因 景宁木兰 

分 类 号：Q75[生物学—分子生物学] S685[农业科学—观赏园艺]