TopoisomeraseⅡαGene as a Marker for Prognostic Prediction of Hepatocellular Carcinoma:A Bioinformatics Analysis  

作　　者：Jin Lu Shaoguang An Junjie Ma Yue Yang Lei Zhang Peng Yu Heng Tao Yunfan Chen Haoxuan Zhang 

机构地区：[1]School of Basic Medicine,Bengbu Medical College,Bengbu,Anhui 233030,China [2]Anhui Key Laboratory of Computational Medicine and Intelligent Health,Bengbu Medical College,Bengbu,Anhui 233030,China [3]Clinical Medical College,Bengbu Medical College,Bengbu,Anhui 233030,China [4]Department of Otolaryngology,First Affiliated Hospital,Bengbu Medical College,Bengbu,Anhui 233030,China [5]Department of Surgical Oncology,Second Affiliated Hospital,Bengbu Medical College,Bengbu,Anhui 233030,China

出　　处：《Chinese Medical Sciences Journal》2022年第4期331-339,共9页中国医学科学杂志（英文版）

基　　金：This work was partially supported by the Key Project of Natural Science Research of Education Department of Anhui Province(No.KJ2019A0338);Industry-University Cooperation Project of the Ministry of Education(No.202101160001).

摘　　要：Objective To investigate the expression of topoisomeraseⅡα(TOP2α)in hepatocellular carcinoma(HCC)and its role in predicting prognosis of HCC patients.Methods We used HCC-related datasets in UALCAN,HCCDB,and cBioPortal databases to analyze the expression and mutation of TOP2αand its co-expressed genes in HCC tissues.GO function and KEGG pathway enrichment of TOP2αand its co-expressed genes were identified.The TIMER database was used to analyze infiltration levels of immune cells in HCC.The impacts of TOP2αand its co-expression genes and the infiltrated immune cells on the survival of HCC patients were assayed by Kaplan-Meier plotter analysis.Results TOP2αand its co-expression genes were highly expressed in HCC(P<0.001)and detrimental to overall survival of HCC patients(P<0.001).TOP2αand its co-expression genes were mainly involved in cell mitosis and proliferation,and cell cycle pathway(ID:hsa04110,P=0.001945).TOP2αand its co-expression genes were mutated in HCC and the mutations were significantly detrimental to overall survival(P=0.0247)and disease-free survival(P=0.0265)of HCC patients.High TOP2αexpression was positively correlated with the infiltration of B cell(r=0.459,P<0.01),CD8^(+)T cell(r=0.312,P<0.01),CD4^(+)T cell(r=0.370,P<0.01),macrophage(r=0.459,P<0.01),neutrophil(r=0.405,P<0.01),and dendritic cell(r=0.473,P<0.01)in HCC.The CD8^(+)T cell infiltration significantly prolonged the 3-and 5-year survival of HCC patients(all P<0.05),and CD4^(+)T cell infiltration significantly shortened the 3-,5-,and 10-year survival of HCC patients(all P<0.05).Conclusion TOP2αmay be an oncogene,which was associated with poor prognosis of HCC patients and could be used as a biomarker for the prognostic prediction of HCC.

关 键 词：topoisomeraseⅡα disease-free survival overall survival hepatocellular carcinoma bioinformatics analysis 

分 类 号：R735.7[医药卫生—肿瘤]