枸杞子中1个新的环香叶烷类单萜  

A new cyclogeraniane monoterpenoid from fruits of Lycium barbarum

作　　者：张文静 范雨欣 胡梦雅 刘云 王小兰[1] 孙彦君[1,2] 李孟 郭涛[1] 冯卫生 陈辉 ZHANG Wen-jing;FAN Yu-xin;HU Meng-ya;LIU Yun;WANG Xiao-lan;SUN Yan-jun;LI Meng;GUO Tao;FENG Wei-sheng;CHEN Hui(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-construction by Henan Province&Ministry of Education,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《中草药》2022年第21期6653-6659,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(22177027):国家自然科学基金资助项目(81860687)。

摘　　要：目的研究宁夏枸杞Lycium barbarum果实(枸杞子)正丁醇部位的化学成分。方法运用D101大孔吸附树脂、硅胶、ODS和Sephadex LH-20等柱色谱以及反相制备型HPLC等各种现代色谱分离技术进行系统的分离纯化,根据化合物的光谱数据和理化性质进行结构鉴定。结果从枸杞子正丁醇部位中分离得到11个化合物,分别鉴定为(3S,5R)-allenic ketodiol 5-O-β-D-glucosyl-(1→3)-β-D-glucoside(1)、citroside B(2)、alangionoside D(3)、turpinionoside D(4)、莨菪亭-7-O-β-D-木糖基-(1→6)-β-D-吡喃葡萄糖苷(5)、2-苯乙醇-β-D-葡萄糖苷(6)、2-苯乙醇-β-纤维二糖苷(7)、tuberonoid A(8)、对羟基肉桂酸(9)、3-甲氧基-4-羟基苯丙酸(10)、阿魏酸(11)。结论化合物1为1个新的环香叶烷类单萜,命名为柑橘苷C。化合物2和3为首次从枸杞属植物中分离得到。体外降血糖活性实验表明,化合物1和4在浓度10.0μmol/L时,可以增加胰岛素抵抗HepG2细胞的葡萄糖消耗量。Objective To isolate and identify the chemical constituents from the n-butanol part of the fruits of Lycium barbarum.Methods The compounds were isolated and purified by various modern chromatographies,and their structures were identified by physiochemical properties and spectroscopic data.Results Eleven compounds were isolated from the n-butanol part of the fruits of L.barbarum,and elucidated as(3S,5R)-allenic ketodiol 5-O-β-D-glucosyl-(1→3)-β-D-glucoside(1),citroside B(2),alangionoside D(3),turpinionoside D(4),scopoletin 7-O-β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside(5),2-phenylethyl-β-D-glucoside(6),2-phenylethylβ-cellobioside(7),tuberonoid A(8),p-hydroxycinnamic acid(9),3-methoxy-4-hydroxyphenylpropionic acid(10)and ferulic acid(11).Conclusion Compound 1 is a new cyclogeraniane monoterpenoid,named citroside C.Compounds 2 and 3 are isolated from Lycium genus for the first time.Compounds 1 and 4 could increase the glucose consumption in insulin-resistant HepG2 cells at a concentration of 10.0μmol/L.

关 键 词：枸杞子 柑橘苷C citroside B turpinionoside D 莨菪亭-7-O-β-D-木糖基-(1→6)-β-D-吡喃葡萄糖苷 2-苯乙醇-β-D-葡萄糖苷 胰岛素抵抗 降血糖活性 

分 类 号：R284.1[医药卫生—中药学]