华中五味子根部木脂素类化学成分及其抗氧化活性研究  

Lignans chemical constituents of roots of Schisandra sphenanthera and their anti-oxidative effects

作　　者：姜侃 郭晔红[1,2] JIANG Kan;GUO Ye-hong(College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;State Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学农学院,甘肃兰州730070 [2]甘肃农业大学省部共建干旱生境作物学国家重点实验室,甘肃兰州730070

出　　处：《中草药》2022年第21期6666-6673,共8页Chinese Traditional and Herbal Drugs

基　　金：甘肃省自然科学基金资助项目(21JR7RA805);甘肃农业大学公招博士科研启动基金项目(GAU-KYQD-2019-01)。

摘　　要：目的 对药食同源植物华中五味子Schisandra sphenanthera根部的化学成分进行研究。方法 利用薄层色谱、柱色谱、半制备高效液相色谱等现代色谱方法分离纯化,根据理化性质及波谱数据对化合物进行结构鉴定;利用ABTS+自由基清除的活性筛选技术对化合物进行抗氧化活性评价。结果 从华中五味子根部70%丙酮水提取物中分离鉴定了23个木脂素类化合物,分别鉴定为(-)-zuoninA (1)、 erythro-1-(4-hydroxy-3-methoxyphenyl)-4-(3,4-methylenedioxyphenyl)-2,3-dimethylbutane(2)、奥斯楚拜脂素-5(3)、9,9′-二羟基-澳白木脂素-5(4)、(-)-8-羟基-α-铁杉脂素(5)、(-)-戈米辛M1(6)、五味子乙素(7)、五味子丙素(8)、戈米辛J(9)、(+)-戈米辛k3(10)、五味子甲素(11)、五味子醇甲(12)、rubrisandrin B(13)、marlignan A(14)、rubrisandrin A(15)、滨南五味子乙素(16)、异戈米辛O(17)、marphenol A(18)、(7′R,8′S)-3,4-dimethoxy-3′,4′-methylenedioxy-7,8-seco-7,7′-epoxylignan-7,8-dione(19)、(7′R,8′S)-3,4-methylenedioxy-3′,4′-dimethoxy-7,8-seco-7,7′-epoxylignan-7,8-dione(20)、machilolin-A(21)、marphenol B(22)、3′,4′-dimethoxy-benzoicacid-(3′′,4′′-dimethoxyphenyl)-3-oxobutyl ester(23)。结论 化合物6、7、18~20、22为首次从该植物中分离得到,化合物1~5、16、17为首次从五味子属植物中分离得到。化合物2~4、13显示了一定的抗氧化能力,半数抑制浓度(median inhibition concentration,IC50)值分别为23.0、16.5、10.8、16.0μmol/L,其中化合物4的抗氧化活性较好,接近阳性对照维生素C。Objective To study the chemical constituents of the roots of Schisandra sphenanthera. Methods The isolation and purification were carried out by macroporous resin, Sephadex LH-20, silica gel column chromatography, semi-preparative HPLC.Their structures were elucidated on the basis of physicochemical properties and spectroscopic data. The anti-oxidant activity of the compounds was evaluated by ABTS+ radical scavenging method. Results Twenty-three compounds were isolated and elucidated from the roots of S. sphenanthera, their structures were identified as(-)-zuonin A(1), erythro-1-(4-hydroxy-3-methoxyphenyl)-4-(3,4-methylenedioxyphenyl)-2,3-dimethylbutane(2), austrobailignan-5(3), 9,9′-dihydroxy-austrobailignan-5(4),(-)-8-hydroxy-α-conidendrin(5),(-)-gomisin M1(6), schisandrin B(7), schisandrin C(8), gomisin J(9),(+)-gomisin k3(10), deoxyschizandrin(11),γ-schizandrin(12), rubrisandrin B(13), marlignans A(14), rubrisandrin A(15), yunnankadsurin B(16), isogomisin O(17),marphenol A(18),(7′R,8′S)-3,4-dimethoxy-3′,4′-methylenedioxy-7,8-seco-7,7′-epoxylignan-7,8-dione(19),(7′R,8′S)-3,4-methylenedioxy-3′,4′-dimethoxy-7,8-seco-7,7′-epoxylignan-7,8-dione(20), machilolin-A(21), marphenol B(22) and 3′,4′-dimethoxy-benzoicacid-(3′′,4′′-dimethoxyphenyl)-3-oxobutyl ester(23). Conclusion Compounds 6, 7, 18—20, 22 are isolated from S. sphenanthera for the first time. Compounds 1—5, 16 and 17 are isolated from the genus Schisandra for the first time. Compounds 2—4, 13 were more active than other compounds in the antioxidant activity with IC50 value 23.0, 16.5, 10.8 and 16.0 μmol/L,respectively. The antioxidant activity of compound 4 was better, which was closed to the positive control vitamin C.

关 键 词：华中五味子 二芳基丁烷类木脂素 联苯环辛烯类木脂素 7 8-seco-木脂素 抗氧化活性 奥斯楚拜脂素-5 (-)-8-羟基-α-铁杉脂素 

分 类 号：R284.1[医药卫生—中药学]