2型糖尿病患者龈上菌斑致龋菌分布与功能研究  

Distribution and function of cariogenic bacteria in supragingival plaque in patients with type 2 diabetes mellitus

作　　者：李玉姣 王玮 潘雅婷 陈力元 范晓敏 田宇 LI Yujiao;WANG Wei;PAN Yating;CHEN Liyuan;FAN Xiaomin;TIAN Yu(State Key Laboratory of Mili-tary Stomatology,National Clinical Research Center for Oral Diseases,Shaanxi Key Laboratory of Stomatology,Depart-ment of Operative Dentistry and Endodontics,School of Stomatology,the Fourth Military Medical University,Xi’an 710032,China)

机构地区：[1]军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔医学重点实验室第四军医大学口腔医院牙体牙髓病科,陕西西安710032

出　　处：《口腔疾病防治》2023年第5期321-327,共7页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：国家自然科学基金项目(81970929)。

摘　　要：目的探讨2型糖尿病患者口腔微生物中致龋菌群落分布特征与功能基因,提高对2型糖尿病与龋病关系的认识。方法试验组选取空军军医大学第一附属医院内分泌科口腔健康的2型糖尿病患者(10例);正常对照组选取社区人群中口腔健康且不患2型糖尿病受试者(10例)。采集口腔健康的2型糖尿病患者组和正常对照组的龈上菌斑样本,分别进行宏基因组学测序,对变异链球菌、乳酸杆菌、黏性放线菌、白色念珠菌等致龋菌进行生物信息学及统计学分析。结果口腔健康的2型糖尿病患者龈上菌斑样本中变异链球菌、乳酸杆菌、黏性放线菌、白色念珠菌丰度稍低于正常对照组,但差异均无统计学意义(P>0.05)。KEGG Pathway功能代谢差异分析结果显示2型糖尿病患者龈上菌斑微生物的D-精氨酸和D-鸟氨酸代谢、生物膜形成-大肠杆菌代谢、己内酰胺降解、精氨酸生物合成等代谢通路较正常对照组丰富,而硫胺素代谢、硒化合物代谢、丙酮酸代谢等代谢通路较正常对照组降低。结论口腔健康的2型糖尿病患者的致龋菌含量与正常对照组并无明显差异,功能基因差异代谢途径表明精氨酸代谢通路富集,有助于维持口腔微生态坏境的酸碱平衡。Objective To investigate the distribution characteristics and functional genes of cariogenic bacteria in oral microorganisms of patients with type 2 diabetes mellitus and to improve the understanding of the relationship between type 2 diabetes mellitus and dental caries.Methods The experimental group included 10 patients with type 2diabetes treated in the Department of Endocrinology,the First Affiliated Hospital of Air Force Medical University.The normal control group included healthy oral subjects without type 2 diabetes in the community population (10 cases).Samples of supragingival plaque from patients with type 2 diabetes mellitus and normal controls were collected and sequenced.Bioinformatics and statistical analysis of cariogenic bacteria such as Streptococcus mutans,Lactobacillus,Actinomyces viscosus and Candida albicans were carried out.Results There were slightly fewer cariogenic bacteria such as Streptococcus mutans,Lactobacillus,Actinomyces viscosus and Candida albicans in supragingival plaque samples of type2 diabetic patients than in normal controls,but the difference was not statistically significant (P>0.05).The results of KEGG pathway functional metabolic differences showed that the metabolic pathways of D-arginine and D-ornithine metabolism,biofilm formation-Escherichia coli,carolactam degradation and arginine biosynthesis were more abundant in the T2DM group than in the normal control group,while metabolic pathways such as tyrosine metabolism,selenocompound metabolism and pyruvate metabolism showed the opposite trend.Conclusion There was no significant difference in the content of cariogenic microorganisms between type 2 diabetic patients and normal control group.The differential metabolic pathways of the functional genes indicated that an increase in the arginine metabolic pathway was beneficial to the maintenance of acid-base balance in the oral microecological environment.

关 键 词：龋病 2型糖尿病 龈上菌斑 宏基因组学 变异链球菌 乳酸杆菌 黏性放线菌 白色念珠菌 

分 类 号：R78[医药卫生—口腔医学]