miR-451、miR-133a-3p、miR-96在胃癌组织中的表达及意义  

Expression and significance of miR-451,miR-133a-3p,and miR-96 in gastric cancer

作　　者：刘丽丹 蒋先明 应茜茜 乐晓伟 尤光贤 LIU Li-dan;JIANG Xian-ming;YING Xi-xi;LE Xiao-wei;YOU Guang-xian(Department of Gastroenterology,Taizhou Tumor Hospital,Taizhou 317502,Zhejiang,China)

机构地区：[1]台州市肿瘤医院放疗科,浙江台州317502

出　　处：《广东医学》2022年第11期1427-1431,共5页Guangdong Medical Journal

基　　金：温岭市科技计划项目(2019S0180019)。

摘　　要：目的探究miR-451、miR-133a-3p、miR-96表达与胃癌病理特征的相关性。方法取收治93例胃癌患者癌组织、癌旁组织标本进行免疫组化染色,对待测标本中miR-451、miR-133a-3p、miR-96表达进行检测,对比分析不同临床病理特征胃癌组织中miR-451、miR-133a-3p、miR-96表达情况,分析miR-451、miR-133a-3p、miR-96表达在胃癌组织中的相关性。结果胃癌患者癌组织中miR-451、miR-133a-3p表达水平低于癌旁组织,miR-96表达水平高于癌旁组织(P<0.05)。不同性别(男、女)、年龄(>60岁、≤60岁)患者癌组织中miR-451、miR-133a-3p、miR-96表达水平比较,差异无统计学意义(P>0.05)。有淋巴结转移患者miR-451、miR-133a-3p表达水平低于无淋巴结转移患者,miR-96表达水平高于无淋巴结转移患者(P<0.05);与黏膜层浸润患者相比,浆膜层浸润患者miR-451、miR-133a-3p表达水平较低,miR-96表达水平较高(P<0.05);与Ⅰ、Ⅱ期患者相比,Ⅲ、Ⅳ期胃癌患者miR-451、miR-133a-3p表达水平较低,miR-96表达水平较高(P<0.05);与高分化患者相比,中、低分化胃癌患者miR-451、miR-133a-3p表达水平较低,miR-96表达水平较高,与中分化患者相比,低分化胃癌患者miR-451、miR-133a-3p表达水平较低,miR-96表达水平较高(P<0.05)。胃癌组织中miR-451、miR-133a-3p表达水平呈正相关(r=0.262,P=0.011);miR-451、miR-96表达水平负相关(r=-0.228,P=0.028);miR-133a-3p、miR-96表达水平呈负相关(r=-0.301,P=0.003)。结论miR-451、miR-133a-3p、miR-96在胃癌中表达出现异常,且miR-451、miR-133a-3p、miR-96表达水平变化与胃癌患者淋巴结转移情况、浸润程度、TNM分期、分化程度相关,三者表达水平之间具有一定相关性,共同参与胃癌发展演进过程。Objective To analyze the correlation between the expression of miR-451,miR-133 a-3 p and miR-96 and the pathological features of gastric cancer.Methods Immunohistochemistry staining of cancer tissues and adjacent noncancerous tissues from 93 patients with gastric cancer were performed to determine the expression of miR-451,miR-133 a-3 p,and miR-96.The expression profiles of miR-451,miR-133 a-3 p,and miR-96 in gastric cancer tissues with different clinicopathological characteristics were analyzed.Results miR-451 and miR-133 a-3 p expression was significantly lower and miR-96 expression was significantly higher in cancer tissues than that in adjacent noncancerous tissues(P<0.05).There was no significant difference in miR-451,miR-133 a-3 p,or miR-96 expression between cancer tissues of male and female,or between patients aged>60 years and those aged≤60 years(P>0.05).Patients with lymph node metastasis had significantly lower mir-451 and mir-133 a-3 p expression than patients without lymph node metastasis and higher miR-96 expression than patients without lymph node metastasis(P<0.05).Patients with serosal layer invasion had significantly lower miR-451 and miR-133 a-3 p expression compared with patients with mucosal layer invasion(P<0.05).Patients with Stage Ⅲ or Ⅳ gastric cancer had significantly lower miR-451 and miR-133 a-3 p expression,and significantly higher miR-96 expression compared with patients with Stage Ⅰ or Ⅱ gastric cancer(P<0.05).Patients with moderately and poor differentiated gastric cancer had significantly lower miR-451 and miR-133 a-3 p expression compared with patients with well and moderate differentiated disease(P<0.05).In gastric cancer,miR-451 and miR-133 a-3 p were positively correlated(r=0.262,P=0.011);miR-451 and miR-96 expression were negatively correlated(r=-0.228,P=0.028);and miR-133 a-3 p and miR-96 expression was negatively correlated(r=-0.301,P=0.003).Conclusion Aberrant expression of miR-451,miR-133 a-3 p,and miR-96 is observed in gastric cancer,correlated with lymph node

关 键 词：胃癌 癌组织 淋巴结转移 浸润程度 病理分期 分化程度 

分 类 号：R735.2[医药卫生—肿瘤] R730.4[医药卫生—临床医学]