发酵法岩藻多糖的硫酸化及其抗氧化活性研究  

On Sulfated Modification and Anti-oxidant Activity of Fucose-Containing Exopolysaccharide from Microbial Fermentation

作　　者：谢爱卿 杨海川[2] 徐兴然[1] 邹祥[1] XIE Aiqing;YANG Haichuan;XU Xingran;ZOU Xiang(College of Pharmaceutical Sclences,Southwest University,Chongqing 400715,China;Yaopharma Co.Ltd.,Chongqing 401121,China)

机构地区：[1]西南大学药学院,重庆400715 [2]重庆药友制药有限责任公司,重庆401121

出　　处：《西南师范大学学报（自然科学版）》2023年第2期65-72,共8页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：重庆市社会事业与民生保障科技创新专项(cstc2016shmszx0184).

摘　　要：岩藻多糖在抗氧化、抗肿瘤和抗病毒方面具有广泛的生物活性,但其在褐藻中含量低且产量少.微生物来源的岩藻多糖与海藻来源的岩藻多糖具有类似的分子结构,但其生理活性不足.本研究对发酵法生产的岩藻多糖进行硫酸化改性以增强其抗氧化活性,使其成为一种可选的岩藻多糖替代品,采用氯磺酸-吡啶法进行硫酸化修饰,红外光谱和核磁氢谱结果显示硫酸基修饰成功,硫酸基含量为28.2%;硫酸化岩藻糖多糖羟基自由基清除率可达81.61%,超氧阴离子清除率可达93.06%;细胞试验表明硫酸化岩藻多糖在50μg/mL及以下无细胞毒性,且低质量浓度(5μg/mL)下即可对RAW 264.7细胞氧化损伤具有保护作用.Fucoidan has a wide range of biological activities in antioxidant,antitumor and antiviral,whearas its content and yield are low in brown algae resources.Compared to fucoidan,fucose-containing exopolysaccharide(FucoPol)from microbial fermentation have similar molecular structures,but lower physiological activity.In this study,fucose-containing polysaccharides was modified by sulfate in order to enhance its antioxidant activity.After sulfidation modification by chlorosulfonic acid-pyridine method,the results of IR and 1HNMR showed that the sulfate group was successfully added,and the content of sulfate group in sulfated FucoPol reached 28.2%;The sulfated fucose-containing exopolysaccharide hydroxyl radical scavenging ability reached 81.61%,and superoxide anions scavenging ability reached 93.06%;Sulfated FucoPol has no obvious cytotoxicity in test concentration,and it can protect RAW 264.7 cells from oxidative damage at low concentration(5μg/mL).Sulfated fucose-containing exopolysaccharide can be used as an alternative substitute of fucoidan.

关 键 词：岩藻多糖 硫酸化 抗氧化 微生物多糖 结构验证 

分 类 号：Q939.9[生物学—微生物学] TS201.2[轻工技术与工程—食品科学]