子宫内膜癌组织DBC1与SIRT1蛋白表达及意义  

PROTEIN EXPRESSION OF DELETED IN BREAST CANCER 1AND SILENT INFORMATION REGULATOR 1IN ENDOMETRIAL CARCINOMA AND RELATED CLINICAL SIGNIFICANCE

作　　者：赵舸 邹存华[1] 宋冬冬[2] 赵淑萍[1] ZHAO Ge;ZOU Cunhua;SONG Dongdong;ZHAO Shu-ping(Gynecological Center,Qingdao Women and Children’s Hospital Affiliated to Qingdao University,Qingdao 266034,China)

机构地区：[1]青岛大学附属青岛市妇女儿童医院妇科中心,山东青岛266034 [2]青岛大学附属青岛市妇女儿童医院普通外科,山东青岛266034

出　　处：《青岛大学学报（医学版）》2022年第6期915-919,共5页Journal of Qingdao University(Medical Sciences)

基　　金：青岛市民生科技计划项目(19-6-49-nsh)。

摘　　要：目的 探讨乳癌缺失因子1(DBC1)蛋白和沉默信息调节因子1(SIRT1)蛋白在子宫内膜癌中的表达及其临床意义,并分析二者在子宫内膜癌中的相关性。方法 收集手术切除的60例子宫内膜癌标本、40例不典型增生子宫内膜标本以及40例同时期因子宫肌瘤手术切除的子宫内膜标本,正常子宫内膜标本40例设为对照组。应用免疫组织化学染色法检测3种类型组织DBC1及SIRT1蛋白的表达情况,同时分析子宫内膜癌中两种蛋白表达的相关性。结果 DBC1蛋白的阳性表达率在子宫内膜癌(70.0%)、不典型增生组织(65.0%)及对照组子宫内膜(37.5%)组织中依次递减,差异有统计学意义(χ^(2)=11.260,P<0.05)。DBC1蛋白的表达与子宫内膜癌的肌层浸润深度(χ^(2)=8.733,P<0.05)、临床分期(χ^(2)=5.833,P<0.05)、病理分级(χ^(2)=9.878,P<0.05)及淋巴结转移有关(χ^(2)=5.945,P<0.05),与病人年龄无关(χ^(2)=1.873,P>0.05)。SIRT1蛋白的阳性表达率在子宫内膜癌(65.0%)、不典型增生组织(62.5%)及对照组子宫内膜(30.0%)组织中逐渐降低,差异有统计学意义(χ^(2)=13.370,P<0.05)。SIRT1蛋白的表达与子宫内膜癌临床分期(χ^(2)=5.910,P<0.05)、病理分级(χ^(2)=8.308,P<0.05)、肌层浸润深度(χ^(2)=10.972,P<0.05)及淋巴结转移有关(χ^(2)=5.918,P<0.05),而与病人年龄无关(χ^(2)=0.188,P>0.05)。相关性分析表明,子宫内膜癌中DBC1蛋白和SIRT1蛋白表达呈正相关(r=0.358,P<0.05)。结论 DBC1和SIRT1的表达可能导致子宫内膜癌的进一步恶化,二者可能成为子宫内膜癌新的治疗靶点。Objective To investigate the protein expression of deleted in breast cancer 1(DBC1) and silent information regulator 1(SIRT1) in endometrial carcinoma and related clinical significance, as well as the correlation between them in endometrial carcinoma. Methods A total of 60 specimens of endometrial carcinoma tissue and 40 specimens of endometrial tissue with atypical hyperplasia were collected, and 40 endometrial specimens from patients undergoing hysterectomy due to hysteromyoma during the same period of time were collected as control group. Immunohistochemistry was used to measure the protein expression of DBC1 and SIRT1 in the three types of endometrial tissue, and the correlation between DBC1 with SIRT1 in endometrial carcinoma was analyzed. Results Endometrial carcinoma tissue showed the highest positive expression rate of DBC1(70.0%), followed by atypical hyperplasia tissue(65.0%) and endometrial tissue in the control group(37.5%), and there was a significant difference between the three groups(χ^(2)=11.260,P<0.05). The protein expression of DBC1 was associated with the depth of myometrial invasion(χ^(2)=8.733,P<0.05), clinical stage(χ^(2)=5.833,P<0.05), pathological grade(χ^(2)=9.878,P<0.05), and lymph node metastasis(χ^(2)=5.945,P<0.05) of endometrial carcinoma, while it was not associated with age(χ^(2)=1.873,P>0.05). Endometrial carcinoma tissue showed the highest positive expression rate of SIRT1(65.0%), followed by atypical hyperplasia tissue(62.5%) and endometrial tissue in the control group(30.0%), and there was a significant difference between the three groups(χ^(2)=13.370,P<0.05). The protein expression of SIRT1 was associated with clinical stage(χ^(2)=5.910,P<0.05), pathological grade(χ^(2)=8.308,P<0.05), the depth of myometrial invasion(χ^(2)=10.972,P<0.05), and lymph node metastasis(χ^(2)=5.918,P<0.05) of endometrial carcinoma, while it was not associated with age(χ^(2)=0.188,P>0.05). Correlation analysis showed that the protein expression of DBC1 was positively correlated with that

关 键 词：子宫内膜肿瘤 乳癌缺失因子1 沉默信息调节因子1 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]