微小RNA⁃375在急性胰腺炎患者外周血中的表达及与并发急性肝损伤的相关性  

Expression of microRNA⁃375 in peripheral blood of patients with acute pancreatitis and its correlation with concurrent acute liver injury

作　　者：陈映群[1] 张卫星[1] 尹子涵 林旭伟 CHEN Yingqun;ZHANG Weixing;YIN Zihan;LIN Xuwei(Intensive Care,Peking University Shenzhen Hospital,Shenzhen,Guangdong,China,518000)

机构地区：[1]北京大学深圳医院重症医学科,广东深圳518000

出　　处：《分子诊断与治疗杂志》2022年第12期2150-2153,2158,共5页Journal of Molecular Diagnostics and Therapy

基　　金：创伤、烧伤与复苏国家重点实验室开放基金项目(SKLKF202011)。

摘　　要：目的探讨急性胰腺炎(AP)患者外周血中微小RNA⁃375(miRNA⁃375)表达及与并发急性肝损伤(ALI)的相关性。方法选取2020年1月至2021年12月北京大学深圳医院AP患者146例为观察组,同期选取健康体检者45名为对照组。比较两组血清miRNA⁃375水平及不同病情AP患者血清miRNA⁃375、淀粉酶水平、急性生理学及慢性健康状况评分系统(APACHEⅡ)评分、Ranson评分、改良CT严重指数(MCTSI),分析AP患者血清miRNA⁃375水平与淀粉酶水平、APACHEⅡ评分、Ranson评分、MCTSI指数相关性,二元Logistic回归分析AP患者并发ALI的影响因素,受试者工作特征(ROC)分析miRNA⁃375对AP患者并发ALI的预测价值。结果观察组血清miRNA⁃375水平高于对照组,差异有统计学意义(P<0.05);重型AP(SAP)患者血清miRNA⁃375、淀粉酶水平、APACHEⅡ评分、Ranson评分、MCTSI指数高于轻型AP(MAP)患者,差异均有统计学意义(P<0.05);AP患者血清miRNA⁃375水平与淀粉酶水平、APACHEⅡ评分、Ranson评分、MCTSI指数均呈正相关(P<0.05);二元Logistic回归分析显示,淀粉酶、miRNA⁃375升高为AP患者并发ALI的独立危险因素(P<0.05);ROC曲线显示,miRNA⁃375预测AP患者并发ALI的AUC为0.810,对应特异度、敏感度分别为0.746、0.791。结论AP患者血清miRNA⁃375异常高表达,与患者病情严重程度有关,且可作为并发ALI的预测标志物。Objective To investigate the expression of microRNA⁃375(miRNA⁃375)in peripheral blood of patients with acute pancreatitis(AP)and its correlation with concurrent acute liver injury(ALI).Methods From January 2020 to December 2021,146 AP patients in Peking University Shenzhen Hospital were selected as the observation group,and 45 healthy subjects were selected as the control group during the same period.The serum miRNA⁃375 levels of the two groups,serum miRNA⁃375,amylase levels,acute physiology and chronic health status scoring system(APACHEⅡ)score,Ranson score,and modified CT severity index(MCTSI)in AP patients with different conditions were compared.The correlation between miRNA⁃375 level and amylase level,APACHE II score,Ranson score,MCTSI index,binary logistic regression analysis of the influencing factors of AP patients complicated with ALI were analyzed.The receiver operating characteristic(ROC)analysis was drawn to evaluate the effect of miRNA⁃375 on AP patients complicated with ALI predicted value.Results The serum miRNA⁃375 level in the observation group was higher than that in the control group,and the difference was statistically significant(P<0.05).Serum miRNA⁃375,amylase level,APACHEⅡscore,Ranson score and MCTSI index in patients with severe AP(SAP)were higher than those in mild AP.AP(MAP)patients,the difference was statistically significant(P<0.05).Serum miRNA⁃375 level in AP patients was positively correlated with amylase level,APACHEⅡscore,Ranson score,MCTSI index(P<0.05).Binary Logistic Regression analysis showed that elevated amylase and miRNA⁃375 were independent risk factors for AP patients complicated with ALI(P<0.05).The ROC curve showed that the AUC of miRNA⁃375 in predicting AP patients with ALI was 0.810,and the corresponding specificity and sensitivity were 0.746 and 0.791,respectively.Conclusion The abnormally high expression of serum miRNA⁃375 in AP patients is related to the severity of the disease and can be used as a predictive marker for concurrent ALI.

关 键 词：急性胰腺炎 微小RNA⁃375 病情 急性肝损伤 

分 类 号：R576[医药卫生—消化系统]