不同生长阶段的长柄扁桃叶蛋白质组学分析及有机酸代谢通路研究  

Proteomics and Organic Acid Metabolic Pathways in Amygdalus Pedunculata Pall Leaves at Different Growth Periods

作　　者：李婷婷 贺玥玥 张晶晶[2] 秦芳玲[3] 李聪[1] 陈邦[1] 申烨华[1] LI Ting-Ting;HE Yue-Yue;ZHANG Jing-Jing;QIN Fang-Ling;LI Cong;CHEN Bang;SHEN Ye-Hua(Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education,College of Chemistry and Materials Science,Northwest University,Xi′an 710127,China;School of Chemical Engineering,Northwest University,Xi′an 710069,China;College of Chemistry and Chemical Engineering,Xi′an Shiyou University,Xi′an 710065,China)

机构地区：[1]西北大学化学与材料科学学院,合成与天然功能分子教育部重点实验室,西安710127 [2]西北大学化工学院,西安710069 [3]西安石油大学化学化工学院,西安710065

出　　处：《分析化学》2023年第1期72-83,I0007-I0009,共15页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21675125);陕西省科技计划项目(Nos.2018ZDXM-NY-087,2019TSLNY03-02)资助。

摘　　要：以长柄扁桃叶片为研究对象,基于蛋白质组学分析,探索了不同生长阶段的长柄扁桃叶片中蛋白质的种类、含量和代谢途径的变化规律。研究结果显示,通过TMT标记定量蛋白质组学检测出6584种可信蛋白,筛选得到1678种差异蛋白。GO功能富集结果表明,差异蛋白参与了防御反应、对生物刺激的反应等生物过程,主要具有信号受体活性、蛋白磷酸酶抑制剂活性、谷胱甘肽转移酶活性和谷胱甘肽脱氢酶(抗坏血酸)活性等分子功能。从开花期到果实成熟期,长柄扁桃叶片中有机酸类化合物的含量逐渐下降,通过KEGG分析发现其主要与α-亚麻酸代谢、乙醛酸和二羧酸代谢以及光合生物中的碳固定这3条代谢通路有关,创伤酸、酮戊二酸、苹果酸和柠檬酸及12种相关蛋白对不同生长期长柄扁桃叶中有机酸类化合物的代谢过程具有重要作用。Based on proteomic analysis,the changes of protein species,content and metabolic pathway in the leaves of Amygdalus pedunculata Pall at different growth periods were investigated and clarified.The results showed that 6584 credible proteins were detected by tandem mass tags(TMT)labeled quantitative proteomics,and 1678 differential proteins were screened.The results of GO functional enrichment showed that the differential proteins participated in the biological processes such as defense response and response to biological stimulation,and mainly had molecular functions such as signal receptor activity,protein phosphatase inhibitor activity,glutathione transferase activity and glutathione dehydrogenase(ascorbic acid)activity.The content of organic acids in Amygdalus pedunculata Pall leaves decreased gradually from flowering stage to fruit ripening stage.Lyoto Encyclopedia of Genes and Genomes(KEGG)analysis showed that it was mainly related toα-linolenic acid metabolism,glyoxylic acid and dicarboxylic acid metabolism and carbon fixation in photosynthetic organisms.Traumatic acid,ketoglutarate,malic acid,citric acid and 12 related proteins played important roles in the metabolic process of organic acids in the leaves of Amygdalus pedunculata Pall at different growth stages.

关 键 词：长柄扁桃叶 蛋白质组学 代谢通路 有机酸 

分 类 号：S662.9[农业科学—果树学]