薏苡仁及其组分对佐剂性关节炎大鼠抗炎镇痛的作用及其机制  

Antiinflammatory and analgesic effects and mechanisms of coix seed and its components in adjuvant arthritis rats

作　　者：岳静 王世军 Yue Jing;Wang Shijun(Shandong Second People’s Hospital,Jinan 250021,Shandong Province,China;College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong Province,China)

机构地区：[1]山东省第二人民医院,山东省济南市250021 [2]山东中医药大学中医学院,山东省济南市250355

出　　处：《中国组织工程研究》2023年第26期4187-4192,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家科学技术部“973”计划项目(2013CB531803),项目负责人:王世军。

摘　　要：背景:类风湿性关节炎是一种自身免疫性疾病,以关节炎症及疼痛为主要症状,属中医痹症范畴。以薏苡仁为主的方剂为除痹经典方,但起效组分及其机制尚不清楚。目的:探讨观薏苡仁及其组分对佐剂性关节炎大鼠的抗炎作用及其作用机制,寻找薏苡仁抗炎的关键组分。方法:64只SPF级Wistar大鼠随机分为正常组、模型组、地塞米松组、薏苡仁水煎液组、薏苡仁挥发油组、薏苡仁蛋白组、薏苡仁多糖组、薏苡仁淀粉组,除正常组外,其余各组大鼠右后足足跖皮下注射完全弗氏佐剂0.2 mL诱导佐剂性关节炎模型,造模后第14天开始灌胃给予相应药物;对照组、模型组给予同体积生理盐水,连续给药18 d。观察各组大鼠一般情况和体质量变化,周长法测量足肿胀度,检测胸腺和脾指数,酶联免疫吸附法检测外周血清白细胞介素1、白细胞介素6和肿瘤坏死因子α质量浓度,苏木精-伊红染色法观察大鼠膝关节病理改变。结果与结论:①与正常组相比,造模后各组大鼠的关节肿胀程度达到高峰期,且体质量显著升高(P<0.05);与模型组比较,薏苡仁水煎液组、薏苡仁挥发油组大鼠体质量显著降低(P<0.05);②与模型组比较,地塞米松组、薏苡仁挥发油组大鼠关节周长均明显下降,关节肿胀程度减轻(均P<0.05);同时地塞米松组、薏苡仁蛋白组及薏苡仁挥发油组大鼠痛阈明显提高(P<0.05);③与正常组相比,模型组大鼠的胸腺指数和脾指数显著升高(P<0.05,P<0.01);与模型组相比,地塞米松能显著降低大鼠胸腺指数(P<0.05)及脾指数(P<0.01),且薏苡仁蛋白可降低大鼠脾指数(P<0.05);④与模型组相比,地塞米松组、薏苡仁蛋白组和薏苡仁挥发油组大鼠血清中白细胞介素1、白细胞介素6和肿瘤坏死因子α质量浓度均显著降低(P<0.01);⑤提示薏苡仁蛋白和薏苡仁挥发油对大鼠类风湿关节炎的抗炎镇痛效果较为显著,可能通�BACKGROUND:Rheumatoid arthritis is an autoimmune disease that causes joint inflammation and pain,which is categorized as arthralgia in traditional Chinese medicine.Coix seed-based prescri ption is the classic prescri ption for relieving arthralgia,but the active components and their mechanisms are still unclear.OBJECTIVE:To investigate the anti-inflammato ry effect and mechanism of coix seed and its components in adjuvant arthritis rats and to find the key antiinflammatory components of coix seed.METHODS:Sixty-four SPF Wistar rats were randomly divided into normal group,model group,dexamethasone group,coix seed decoction group,coix seed volatile oil group,coix seed protein group,coix seed polysaccharide group,and coix seed starch group.In addition to the normal group,animal models were successfully established in the other groups by subcutaneous injection of 0.2 mL of Freund’s complete adjuvant into the planta pedis of the right hindfoot.Drug administration by gavage began on the 14thday after modeling and lasted for 18 days.The control and model groups were given the same volume of normal saline.General conditions and body mass changes in rats were observed.The swelling degree of the feet was measured by pe rimeter method.Thymus and spleen indexes were detected.Levels of inte rleukin-1,interleukin-6,and tumor necrosis factor α in the ca rtilage were detected by enzyme-linked immunosorbent assay.Histopathological changes of rat knee joint were observed by hematoxylin-eosin staining.RESULTS AND CONCLUSION:Compared with the normal group,the degree of joint swelling in each group reached the peak after modeling(14 days),and the body mass was significantly increased(P<0.05).Compared with the model group,the body mass of rats in coix seed decoction group and coix seed volatile oil group decreased significantly(P<0.05).Compared with the model group,the joint circumfe rence of rats in the dexamethasone group and coix seed volatile oil group decreased significantly,and the degree of joint swelling decreased(both P<0.0

关 键 词：薏苡仁 有效组分 佐剂性关节炎 动物模型 炎性因子 大鼠 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R259