半红树林植物黄槿内生真菌多样性及其抗菌活性研究  

Study on diversity and antimicrobial activity of endophytic fungi isolated from semi-mangrove plant Hibiscus tiliaceus

作　　者：蒋圆婷 陈文凤 田婧[1] 刘艳丽[1] 陈德力[2,3] 梁寒峭 JIANG Yuanting;CHEN Wenfeng;TIAN Jing;LIU Yanli;CHEN Deli;LIANG Hanqiao(Department of Biomedicine,Beijing City University,Beijing 100083;Hainan Branch Institute of Medicinal Plant Development(Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine),Chinese Academy of Med-ical Sciences&Peking Union Medical College,Haikou 570311;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193)

机构地区：[1]北京城市学院生物医药学部,北京100083 [2]中国医学科学院北京协和医学院药用植物研究所海南分所,海南省南药资源保护与开发重点实验室,海口570311 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《安徽农业大学学报》2022年第5期838-847,共10页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(21808004);北京城市学院科研发展基金(KYF202003);北京城市学院2022年度研究生科研创新项目(Yjscx202243)共同资助。

摘　　要：黄槿植物的叶、树皮和花是中药黄槿的入药部位,具有抗菌、抗炎、抗肿瘤的药用价值。药用植物内生真菌与宿主植物在基因层面存在信息传递,会产生与宿主植物相同或相似的代谢产物,因此黄槿内生真菌次生代谢产物可能成为新型天然活性产物。从不同地点采集的半红树林植物黄槿中分离纯化出64株内生真菌并鉴定,通过固体发酵得到其次生代谢产物,采用牛津杯法检测内生真菌的粗提物对5种常见致病细菌的抑菌活性,后根据分离部位的不同构建系统发育树,并筛选确定3株具有强抑菌活性的菌株,用于后续次生代谢产物中化学成分的研究。The leaves,barks and flowers of Hibiscus tiliaceus are the medicinal parts of the traditional Chinese medicine H.tiliaceus,which have the medicinal values of antibacterial,anti-inflammatory and anti-tumor.There is information transmission between medicinal plant endophytic fungi and host plants at the genetic level,which can produce the same or similar metabolites as host plants,so H.tiliaceus endophytic fungi may become one of the sources of new natural products.In this study,64 strains of endophytic fungi were isolated and identified from different parts of semi-mangrove plant H.tiliaceus,fermented in solid state,and then oxford cup method was used to detect the bacteriostatic activities of the metabolites to five kinds of common pathogenic bacteria.According to different construction of phylogenetic tree of the separate parts,three strains had been screened to be inhibitor activity of bacteria,which can be used to study the chemical composition of metabolites in the future.

关 键 词：黄槿内生真菌 次生代谢产物 抑菌活性 系统发育分析 

分 类 号：R282.71[医药卫生—中药学] Q939.92[医药卫生—中医学]