适配体识别-化学发光技术检测福氏志贺菌  

Detection of Shigella frigneri based on aptamer recognition and chemiluminescence technique

作　　者：陈威风 李晓雪 裴兰梅 常惟丹 崔丽伟[1] 潘春梅[1] CHEN Weifeng;LI Xiaoxue;PEI Lanmei;CHANG Weidan;CUI Liwei;PAN Chunmei(School of Food and Bioengineering,Henan university of animal husbandry and economy,Zhengzhou 450046,China)

机构地区：[1]河南牧业经济学院食品与生物工程学院,河南郑州450046

出　　处：《食品与发酵工业》2023年第1期267-272,278,共7页Food and Fermentation Industries

基　　金：河南省科技攻关项目(212102310259);河南省高等学校重点科研项目(22B550003,22B150005)。

摘　　要：福氏志贺菌(Shigella flexneri)是一种危害人类健康的食源性致病菌。该实验首先制备鲁米诺功能化花状纳米金和氨基化磁性纳米Fe_(3)O_(4),然后将福氏志贺菌适配体的互补序列(FSZ-2HH)连接在鲁米诺功能化花状纳米金的表面构建信号探针,并将福氏志贺菌适配体(FSZ-1)通过亲和素的桥接作用修饰到氨基化磁性纳米Fe_(3)O_(4)表面构建捕获探针。当目标物存在时,目标物和信号探针会竞争性结合捕获探针,进而通过测定化学发光来达到检测目的。实验结果表明:在浓度2.6×10~2.6×10^(5) CFU/mL,福氏志贺菌的浓度与化学发光强度呈良好的线性关系,检出限为12 CFU/mL,特异性良好。该实验建立的方法为食品中福氏志贺菌的快速高灵敏检测提供了良好思路。Shigella flexneri is a foodborne pathogen highly threatening human health.Firstly,luminol functionalized flower-like gold nanoparticles and amino magnetized nano-Fe_(3)O_(4) were prepared.Afterward,the complementary sequence of S.flexneri aptamer(FSZ-2HH)was connected to the surface of the luminol functionalized flower-like gold nanoparticles to construct a signal probe whereas the S.flexneri aptamer(FSZ-1)was modified to the amino magnetic nano-Fe_(3)O_(4) surface by avidin bridging to construct a capture probe.When the Shigella flexneri is present,it will compete with the signal probe for binding the capture probe,whereby the bacteria could be detected via measuring the chemiluminescence.The results showed that in the range of 2.6×10^(1) to 2.6×10^(5) CFU/mL,there was a good linear relationship between the concentration of S.flexneri and the chemiluminescence intensity,with good specificity and a detection limit of 12 CFU/mL.This method provides a potential tool for the rapid and sensitive detection of S.flexneri in foods.

关 键 词：福氏志贺菌 适配体传感器 化学发光 磁分离技术 

分 类 号：TS207.4[轻工技术与工程—食品科学] O657.3[轻工技术与工程—食品科学与工程]