嘌呤能受体P2X3在急性牙髓炎大鼠疼痛中的作用  

Role of Purinergic Receptor P2X3 in Pain of Acute Dental Pulp Inflammation of Rat

作　　者：陈杨曦 胡君 黄丽丽 康逸群 王丽[1,3] CHEN Yangxi;HU Jun;HUANG Lili;KANG Yiqun;WANG Li(The State Key Laboratory Breeding Base of Basic Science of Stomatology,Hubei Province&Key Laboratory of Oral Biomedicine(Wuhan University),Ministry of Education,Wuhan 430079,China;Department of Emergency and General Dentistry,School and Hospital of Stomatology,Wuhan University,Wuhan 430079,China;Department of Anesthesiology,School and Hospital of Stomatology,Wuhan University,Wuhan 430079,China)

机构地区：[1]口腔医学湖北省重点实验室-省部共建国家重点实验室培育基地,口腔生物医学教育部重点实验室(武汉大学),湖北武汉430079 [2]武汉大学口腔医院综合急诊科,湖北武汉430079 [3]武汉大学口腔医院麻醉科,湖北武汉430079

出　　处：《口腔医学研究》2023年第1期24-31,共8页Journal of Oral Science Research

基　　金：国家自然科学基金青年项目(编号:82001073、82100957);湖北省自然科学基金面上项目(编号:2020CFB621)。

摘　　要：目的:探讨实验诱导的急性牙髓炎动物模型中,嘌呤能受体P2X3参与调控牙髓炎大鼠疼痛中的作用。方法:本实验通过牙髓暴露和脂多糖的应用在Wistar大鼠中诱导急性牙髓炎。SHAM组大鼠仅接受相同的麻醉过程,但没有任何其他干预措施。牙髓炎组大鼠分别于术后第1天和第3天,记录大鼠的擦面行为次数和测量头部缩回反射阈值,评估每组大鼠牙髓炎引起的疼痛。然后对大鼠实施安乐死,对三叉神经节、脑干的三叉神经脊束核尾端亚核和丘脑的腹后中核进行观察分析。另取两组牙髓炎大鼠分别给与P2X3受体特异性拮抗剂A-317491,于术后第1天和第3天,再次评估大鼠的擦面行为次数和测量头部缩回反射阈值。结果:在急性牙髓炎后的第1天和第3天,均发现了严重牙髓炎症的组织学证据,并且大鼠的疼痛样行为显着增加(P<0.05)。急性牙髓炎在术后第1天和第3天均诱导了左侧三叉神经节中P2X3表达上调,左侧三叉神经节中的P2X3免疫反应性显著高于对照组和右侧(P<0.05)。在双侧三叉神经脊束核中,与SHAM组相比,P2X3在术后第1天和第3天表达均没有变化(P>0.05)。在右侧丘脑中,与SHAM组比较,P2X3在术后第3天表达增加(P<0.05),在术后第1天表达没有变化;在左侧丘脑中,P2X3在术后第1天和第3天表达均没有变化(P>0.05);拮抗三叉神经节中的P2X3表达能够减轻大鼠的伤害性反应。结论:在急性牙髓炎症过程中,P2X3受体参与了外周神经系统的伤害性信号处理,拮抗三叉神经节中的P2X3能够减轻大鼠的伤害性反应。牙髓炎没有引起三叉神经脊束中P2X3表达上调,但在右侧丘脑的腹后中核中P2X3的表达有所增加,需要进一步的研究来确定是否有其他的信号通路介导牙髓炎的疼痛信号传导。Objective:To verify the expression of purinergic receptor P2X3 at important sites in the peripheral and central nervous systems and its involvement in the regulation and integration of pain information during experimentally induced pulpitis.Methods:Acute pulpitis was induced by the pulp exposure and lipopolysaccharide application in Wistar rats.Animals in the SHAM group received the same anesthetic procedure without any other interventions.On the first and third postoperative days,face-grooming activity and the measurement of head retraction reflex thresholds were recorded to assess the pulpitis-induced pain in each group of rats.The rats were then euthanized to conduct the observational analysis of the trigeminal ganglion(TG),trigeminal spinal subnucleus caudalis(Ⅴc)of the brain stem,and ventral posteromedial nucleus(VPM)of the thalamus.Results:Histological evidence of severe pulp inflammation was observed on day 1 and 3 after tooth injury,and the pain-like behavior of animals showed a significant increase(P<0.05).For acute pulpitis,the upregulation of P2X3 in the ipsilateral TG was induced on the first and third postoperative days.P2X3 immunoreactivity in the left TG was significantly higher than that in the control and right side(P<0.05).In the bilateral trigeminal spinal nucleus,the expression of P2X3 was not changed on the first and third postoperative days compared with that in the SHAM group(P>0.05).In the right thalamus,the expression of P2X3 increased on the third postoperative day but showed no change on the first postoperative day compared with that in the SHAM group.In the left thalamus,the expression of P2X3 exhibited no change on the first and third postoperative days.Conclusion:P2X3 channels were significantly involved in injurious signal processing in the peripheral nervous system following acute pulp inflammation,but probably not involved at the brain stem level.The upregulation of P2X3 expression in theⅤc of the brain stem was not induced by pulpitis.However,the expression of P2X3 increased

关 键 词：口颌面部 疼痛 嘌呤能受体 

分 类 号：R781.31[医药卫生—口腔医学]