湖北省3~12岁汉族儿童神经递质正常参考值范围的建立  

Establishment of the normal reference range of neurotransmitters in Han-nationality children aged 3-12 in Hubei province

作　　者：钱乔乔 曹中强[2] 谈倩倩 周勇 刘杨茜 吴迁 辛莹莹 孙丹[1] 刘智胜[1] Qian Qiaoqiao;Cao Zhongqiang;Tan Qianqian;Zhou Yong;Liu Yangxi;Wu Qian;Xin Yingying;Sun Dan;Liu Zhisheng(Department of Neurology,Wuhan Children′s Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430016,China;Research Institute for Women and Children′s Health,Wuhan Children′s Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430016,China;Wuhan Kindstar Diagnostics Co.,Ltd,Wuhan 430016,China)

机构地区：[1]华中科技大学同济医学院附属武汉儿童医院神经内科,武汉430016 [2]华中科技大学同济医学院附属武汉儿童医院妇女儿童健康研究所,武汉430016 [3]武汉康圣达医学检验所有限公司,武汉430016

出　　处：《中华实用儿科临床杂志》2022年第19期1469-1472,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：2020年度武汉市医学科研项目(WX20A13);首届小贴士杯抽动障碍科研基金项目(CTDSF-2021-03)。

摘　　要：目的建立湖北省3~12岁汉族儿童神经递质正常参考值范围。方法采用前瞻性研究方法, 对2021年1月至8月在武汉儿童医院体检的3~12岁湖北省汉族健康儿童进行病史询问, 排除神经、精神系统疾病及感染、创伤、近2周用药史, 收集324例儿童(男262例, 女62例;3~7岁组217例, 8~12岁组107例)血浆及391例儿童(男302例, 女89例;3~7岁组266例, 8~12岁组125例)随机尿液, 通过超高效液相色谱-质谱联用技术(UPLC-MS/MS)检测受试者血浆中多巴胺、肾上腺素、谷氨酸等10种神经递质及随机尿液中多巴胺、肾上腺素、5-羟基吲哚乙酸等8种神经递质的水平, 建立湖北省3~12岁汉族儿童的神经递质正常参考范围。采用Kruskal-Wallis H检验对不同年龄和性别间神经递质分布水平的差异进行统计分析, 并采用Nemenyi检验进行不同组间的两两比较。结果不同性别儿童中各种神经递质水平差异均无统计学意义(均P>0.05)。血浆中多巴胺、甲氧基去甲肾上腺素、色氨酸、γ氨基丁酸在3~7岁组和8~12岁组差异有统计学意义, 随机尿中多巴胺、肾上腺素、去甲肾上腺素、甲氧基肾上腺素、甲氧基去甲肾上腺素、高香草酸、5羟基吲哚乙酸在3~7岁组和8~12岁组差异有统计学意义。结论建立了湖北省3~12岁汉族儿童神经递质正常参考值范围, 对临床具有重要参考意义, 对儿童神经递质相关疾病的研究奠定了基础。Objective To establish the normal reference range of neurotransmitters in Han-nationality children aged 3-12 in Hubei province.Methods A prospective study was conducted on healthy Han-nationality children aged 3-12 who took physical examination in Wuhan Children′s Hospital,Hubei province from January to August 2021.The children were asked for their medical histories,and those with neurological diseases,psychiatric diseases,infection,trauma,and a drug history in the past 2 weeks were excluded.The plasma of 324 children(262 males,62 females;217 cases in the 3-7 years old,107 cases in the 8-12 years old)and urine of 391 children(302 males,89 females;266 cases in the 3-7 years old,125 cases in the 8-12 years old)were collected.They ultra performance liquid chromatography-mass spectrometry multiple techniques(UPLC-MS/MS)were used to detect 10 kinds of neurotransmitters(e.g.,dopamine,epinephrine,glutamic acid,etc.)in plasma and 8 kinds of neurotransmitters(e.g.,dopamine,epinephrine,5-hydroxyindoleacetic acid,etc.)in random urine.The normal reference range of neurotransmitters in Han-nationality children aged 3-12 in Hubei province was established.The Kruskal-Wallis H test was made for statistical analysis of the differences in neurotransmitter levels among different age groups and gender groups.The neurotransmitter levels between different groups were compared by the Nemenyi test.Results There were no significant differences in the levels of various neurotransmitters in children of different genders(all P>0.05).There were significant differences in the levels of dopamine,methoxy-norepinephrine,tryptophan andγ-aminobutyric acid in the plasma of children aged 3-7 years and 8-12 years.There were significant differences in the levels of dopamine,epinephrine,norepinephrine,methoxy-norepinephrine,high vanillic acid and 5-hydroxyindoleacetic acid in the random urine between the 3-7 years old group and the 8-12 years old group.Conclusions The normal reference range of neurotransmitters in Han-nationality children aged 3-12

关 键 词：儿童 神经递质 参考值范围 

分 类 号：R741[医药卫生—神经病学与精神病学]