表面增强拉曼光谱法快速检测百合中的地虫硫磷  

Rapid detection of fonofos in Lilium brownii var.viridulum Baker by surface enhanced Raman spectroscopy

作　　者：贾莉 马俊 李壮 王淼[1] 潘凌屹 杨艺玥 王猛强 焦逊 肖明[3] 佘永新[1] JIA Li;MA Jun;LI Zhuang;WANG Miao;PAN Ling-Yi;YANG Yi-Yue;WANG Meng-Qiang;JIAO Xun;XIAO Ming;SHE Yong-Xin(Institute of Agricultural Quality Standards and Testing Technology for Aro-products,Chinese Academy of Agricultural Sciences,Beijing 100081,China;National Anti-drug Laboratory Beijing Regional Center,Beijing 100079,China;Academy of Agriculture and Forestry Sciences,Qinghai University,Qinghai 810016,China)

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京100081 [2]国家毒品实验室北京分中心,北京100079 [3]青海大学农林科学院,青海810016

出　　处：《食品安全质量检测学报》2022年第24期7956-7963,共8页Journal of Food Safety and Quality

基　　金：国家重点研发计划项目(2021YFD1600105);中国农业科学院农业科技创新计划项目(Y2021XK21);国家自然科学基金项目(31772071)。

摘　　要：目的制备具有良好增强效果的L-半胱氨酸功能化修饰的金纳米粒子(L-cysteine-Au nanoparticles,L-cys-AuNPs),建立表面增强拉曼光谱法(surface-enhanced Raman spectroscopy,SERS)快速检测百合中地虫硫磷的分析方法。方法采用柠檬酸钠还原法制备Au NPs,然后用L-半胱氨酸进行功能化修饰。通过单因素实验对增强基底添加量、地虫磷添加量、凝聚剂种类等实验条件进行优化,以确定最佳实验条件。结果该基底表现出优异的增强效果,1071 cm^(-1)可作为其特征峰。地虫硫磷的检出限为0.02 mg/L,线性范围是0.02~0.50 mg/L,相关系数r^(2)=0.9667,回收率为69%~112%,相对标准偏差(relative standard deviations,RSDs)为7%~8%。结论该方法灵敏度高、特异性、稳定性较好,可用于百合中地虫硫磷的快速检测。Objective To prepare L-cysteine functionalized gold nanoparticles(L-cysteine-Au nanoparticles,L-cys-AuNPs)with good enhancement effect,and establish a method for the rapid detection of fonofos in Lilium brownii var.viridulum Baker by surface-enhanced Raman spectroscopy(SERS).Methods AuNPs were prepared by sodium citrate reduction and then functionalized with L-cysteine.Then the single factor experiment was conducted to optimize the experimental conditions,such as the amount of enhanced substrate,the amount of fonofos and the type of coagulant,so as to determine the best experimental conditions.Results The substrate showed excellent enhancement effect,and 1071 cm^(-1) could be used as its characteristic peak.The limit of detection was 0.02 mg/L,the linear range was 0.02-0.50 mg/L,the correlation coefficient(r^(2))was 0.9667,the recoveries were 69%-112%,and the relative standard deviations(RSDs)were 7%-8%.Conclusion The method is sensitive,specific and stable,and can be used for the rapid detection of fonofos in Lilium brownii var.viridulum Baker.

关 键 词：L-半胱氨酸 金纳米粒子 表面增强拉曼光谱法 百合 地虫硫磷 

分 类 号：O657.37[理学—分析化学] TS207.53[理学—化学]