Development of a multicellular 3D-bioprinted microtissue model of human periodontal ligament-alveolar bone biointerface:Towards a pre-clinical model of periodontal diseases and personalized periodontal tissue engineering  

作　　者：Murat Taner Vurat ŞükranŞeker Özge Lalegül-Ülker Mahmut Parmaksiz Ayşe Eser Elçin Yaşar Murat Elçin 

机构地区：[1]Tissue Engineering,Biomaterials and Nanobiotechnology Laboratory,Ankara University Faculty of Science,Ankara University Stem Cell Institute,Ankara 06100,Turkey [2]Biovalda Health Technologies,Inc.,Ankara 06100,Turkey

出　　处：《Genes & Diseases》2022年第4期1008-1023,共16页基因与疾病（英文）

基　　金：This work was partially supported by a grant from The Scientific and Technological Research Council of Turkey (TUBITAK;No. 117M281).

摘　　要：While periodontal (PD) disease is among principal causes of tooth loss worldwide, regulation of concomitant soft and mineralized PD tissues, and PD pathogenesis have not been completely clarified yet. Besides, relevant pre-clinical models and in vitro platforms have limitations in simulating human physiology. Here, we have harnessed three-dimensional bioprinting (3DBP) technology for developing a multi-cellular microtissue model resembling PD ligament-alveolar bone (PDL-AB) biointerface for the first time. 3DBP parameters were optimized;the physical, chemical, rheological, mechanical, and thermal properties of the constructs were assessed. Constructs containing gelatin methacryloyl (Gel-MA) and hydroxyapatite-magnetic iron oxide nanoparticles showed higher level of compressive strength when compared with that of Gel-MA constructs. Bioprinted self-supporting microtissue was cultured under flow in a microfluidic platform for >10 days without significant loss of shape fidelity. Confocal microscopy analysis indicated that encapsulated cells were homogenously distributed inside the matrix and preserved their viability for >7 days under microfluidic conditions. Immunofluorescence analysis showed the cohesion of stromal cell surface marker-1+ human PDL fibroblasts containing PDL layer with the osteocalcin+ human osteoblasts containing mineralized layer in time, demonstrating some permeability of the printed constructs to cell migration. Preliminary tetracycline interaction study indicated the uptake of model drug by the cells inside the 3D-microtissue. Also, the non-toxic levels of tetracycline were determined for the encapsulated cells. Thus, the effects of tetracyclines on PDL-AB have clinical significance for treating PD diseases. This 3D-bioprinted multi-cellular periodontal/osteoblastic microtissue model has potential as an in vitro platform for studying processes of the human PDL.

关 键 词：3D bioprinting Alveolar bone Microtissue model Organ-on-a-chip .Periodontal ligament Periodontal tissue engineering Periodontal-osteoblastic biointer face Periodontium-on-a-chip 

分 类 号：R781.4[医药卫生—口腔医学]