BET bromodomain inhibitor JQ1 regulates spermatid development by changing chromatin conformation in mouse spermatogenesis  

作　　者：Xiaorong Wang Mengmeng Sang Shengnan Gong Zhichuan Chen Xi Zhao Guishuan Wang Zhiran Li Yingying Huang Shitao Chen Gangcai Xie Enkui Duan Fei Sun 

机构地区：[1]Institute of Reproductive Medicine,School of Medicine,Nantong University,Nantong,Jiangsu 226001,PR China [2]International Peace Maternity and Child Health Hospital,Shanghai Key Laboratory for Reproductive Medicine,School of Medicine,Shanghai Jiaotong University,Shanghai 200030,PR China [3]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,PR China

出　　处：《Genes & Diseases》2022年第4期1062-1073,共12页基因与疾病（英文）

基　　金：This study was supported by the National Key Research and Development Program of China (No. 2018YFC1003500);the National Natural Science Foundation of China (No. 81901528).

摘　　要：As a BET bromodomain inhibitor, JQ1 has been proven have efficacy against a number of different cancers. In terms of male reproduction, JQ1 may be used as a new type of contraceptive, since JQ1 treatment in male mice could lead to germ cell defects and a decrease of sperm motility, moreover, this effect is reversible. However, the mechanism of JQ1 acting on gene regulation in spermatogenesis remains unclear. Here, we performed single-cell RNA sequencing (scRNA-seq) on mouse testes treated with JQ1 or vehicle control to determine the transcriptional regulatory function of JQ1 in spermatogenesis at the single cell resolution. We confirmed that JQ1 treatment could increase the numbers of somatic cells and spermatocytes and decrease the numbers of spermatid cells. Gene Ontology (GO) analysis demonstrated that differentially expressed genes which were down-regulated after JQ1 injection were mainly enriched in “DNA conformation change” biological process in early developmental germ cells and “spermatid development” biological process in spermatid cells. ATAC-seq data further confirmed that JQ1 injection could change the open state of chromatin. In addition, JQ1 could change the numbers of accessible meiotic DNA double-stranded break sites and the types of transcription factor motif that functioned in pachytene spermatocytes and round spermatids. The multi-omics analysis revealed that JQ1 had the ability to regulate gene transcription by changing chromatin conformation in mouse spermatogenesis, which would potentiate the availability of JQ1 in male contraceptive.

关 键 词：Chromatin conformation JQ1 scRNA-seq Spermatid development SPERMATOGENESIS 

分 类 号：R383.24[医药卫生—医学寄生虫学]