健脾补肾方对辐射损伤小鼠全血细胞及免疫器官的影响研究  

Effect of Jianpi Bushen decoction on whole blood cells and immune organs of radiation injured mice

作　　者：何东初 肖静静 张梦云 张勇 丁晓娟 伍伟 杨文超 HE Dongchu;XIAO Jingjing;ZHANG Mengyun;ZHANG Yong;DING Xiaojuan;WU Wei;YANG Wenchao(General Hospital of Central Theater Command,Wuhan 430070,Hubei,China)

机构地区：[1]中部战区总医院,湖北武汉430070

出　　处：《现代中西医结合杂志》2022年第22期3067-3073,3105,共8页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：国家自然科学基金面上项目(81873153,81273905)。

摘　　要：目的研究健脾补肾方对辐射损伤小鼠全血细胞减少及免疫器官损伤的干预作用。方法取90只昆明种小鼠,随机选择18只作为正常组,其余72只小鼠采用6.0 Gy X射线一次性全身照射。将造模成功小鼠随机分为模型组、健脾补肾方大剂量组、健脾补肾方小剂量组、胸腺肽组,每组18只。健脾补肾方大、小剂量组分别给予生药浓度2 g/mL和1 g/mL健脾补肾方灌胃,胸腺肽组给予胸腺肽肠溶片混悬液0.325 mg/mL灌胃,正常组和模型组给予生理盐水灌胃,均2次/d。每组分别于灌胃第7,14,30天各取6只小鼠称体重,收集外周血,分离脾脏、胸腺,检测外周血白细胞计数、淋巴细胞计数、红细胞计数、血红蛋白含量、血小板计数及脾脏指数、胸腺指数,HE染色观察脾脏组织形态学。结果灌胃第7天,模型组小鼠外周血白细胞计数、淋巴细胞计数、红细胞计数、血红蛋白含量、血小板计数及胸腺指数、脾脏指数均明显低于正常组(P均<0.05),脾脏病理改变明显。灌胃第14天,模型组小鼠外周血红细胞计数、血红蛋白含量、脾脏指数回升,但仍明显低于正常组(P均<0.05);白细胞计数、淋巴细胞计数、血小板计数及胸腺指数仍持续下降,脾脏病理改变略减轻。灌胃第30天,模型组小鼠外周血白细胞计数、淋巴细胞计数、红细胞计数、血红蛋白含量、血小板计数均回升,但仍明显低于正常组(P均<0.05);胸腺指数、脾脏指数大致同前,脾脏病理改变略减轻。健脾补肾方小、中剂量组及胸腺肽组不同时间点外周血白细胞计数、淋巴细胞计数、红细胞计数、血红蛋白含量、血小板计数和脾脏指数、胸腺指数均明显高于模型组(P均<0.05),脾脏病理改变减轻;健脾补肾方小剂量组各指标改善情况优于健脾补肾方大剂量组,促进红细胞计数、血红蛋白含量、血小板计数恢复效果优于胸腺肽组。灌胃第7天,健脾补肾方小�Objective It is to study the therapeutic effect of Jianpi Bushen decoction on pancytopenia and damage of immune organs in mice with radiation injury.Methods Ninety Kunming mice were selected,in which 18 mice were randomly selected as the normal group,and the rest 72 mice were treated with 6.0 Gy X-ray total body irradiation.The successful modeled mice were randomly divided into model group,Jianpi Bushen decoction large dose group,Jianpi Bushen decoction small dose group and thymosin group,with 18 mice in each group.The large and small dose groups of Jianpi Bushen decoction were respectively given Jianpi Bushen decoction whose raw drug concentration were 2 g/mL and 1g/mL by gavage,the thymosin group was given the thymosin enteric-coated tablet suspension 0.325 mg/mL by gavage,the normal group and the model group were given normal saline by gavage,all twice a day.6 mice were taken from each group on the 7 th,14 th and 30th day of gavage,and were weighed.Their peripheral blood was collected,spleen and thymus were separated to detect the white blood cell count,lymphocyte count,red blood cell count,hemoglobin content,platelet count,spleen index and thymus index,and to observe histomorphology of spleen by HE staining.Results On the 7th day of gavage,the peripheral blood white blood cell count,lymphocyte count,erythrocyte count,hemoglobin content,platelet count,thymus index and spleen index of the model group were significantly lower than those of the normal group(all P<0.05),and the pathological changes of spleen were obvious.On the 14 th day of gavage,the peripheral blood red blood cell count,hemoglobin content and spleen index of mice in the model group were increased,but still significantly lower than those in the normal group(all P<0.05);The white blood cell count,lymphocyte count,platelet count and thymus index were continuously decreased,and the pathological changes of spleen were slightly alleviated.On the 30th day of gavage,the peripheral blood white blood cell count,lymphocyte count,red blood cell count,hemogl

关 键 词：健脾补肾方 辐射损伤 全血细胞 免疫器官 

分 类 号：R-332[医药卫生]