上皮细胞损伤对口腔黏膜成纤维细胞胶原分泌的影响  

Effect of epithelial cell injury on collagen secretion of oral mucous fibroblasts

作　　者：张玮 吴建军 ZHANG Wei;WU Jian-jun(Department of Endodontics,Center of Stomatology,Xiangya Hospital,Central South University,Changsha 410008,China)

机构地区：[1]中南大学湘雅医院口腔医学中心牙体牙髓科,长沙410008 [2]中南大学湘雅口腔医院口腔颌面外科

出　　处：《北京口腔医学》2022年第6期407-411,共5页Beijing Journal of Stomatology

摘　　要：目的 探究上皮细胞损伤对口腔黏膜成纤维细胞形态、基因表达及胶原分泌的影响,以此分析在口腔黏膜下纤维性变进程中的作用。方法 成纤维细胞分为A、B两组,两组均使用上皮细胞离心上清液干预,但B组预先将上皮细胞损伤,A组为对照,无特殊处理。于干预后0、24、48 h观察并比较两组成纤维细胞形态,qPCR检测α-SMA、COL1A2和COL3A1表达量,ELISA检测Ⅰ型胶原和Ⅲ型胶原分泌量,并做统计学分析。结果 A组细胞呈梭形,无突起,干预后48 h少部分呈多角形;B组干预后24、48 h细胞呈多角形,突起较多。两组中α-SMA、COL1A2和COL3A1表达量及Ⅰ型胶原和Ⅲ型胶原分泌量,在干预后24、48 h B组均高于A组,且存在统计学差异(P<0.05)。结论 上皮细胞损伤可以使得成纤维细胞变形,增加α-SMA、COL1A2和COL3A1表达,刺激Ⅰ型胶原和Ⅲ型胶原的分泌。上皮细胞损伤可能参与了口腔黏膜下纤维性变的进程。Objective To investigate the effect of epithelial cell injury on the morphology, gene expression and collagen secretion of oral mucous fibroblasts. Methods Fibroblasts were divided into two groups, which were treated with the supernatant after the epithelial cells were centrifuged. In group B, the epithelial cells were pre-injured, group A served as control. The morphology of the fibroblasts was observed and compared 0, 24, and 48 hours after the intervention. The expressions of α-SMA, COL1A2 and COL3A1 were detected with qPCR. The secretions of collagen I and collagen Ⅲ were examined by ELISA. Results The fibroblasts in group A were fusiform without protuberance, but the fibroblasts in group B were polygonal with protrusions, 24 and 48 hours after the intervention. The expressions of α-SMA,COL1A2 and COL3A1 and the secretions of collagen I and collagen Ⅲ in group B were more than that in group A 24 and 48 hours after intervention(P<0.05).Conclusions Epithelial cell injury can deform fibroblasts, increase the expressions of α-SMA,COL1A2 and COL3A1 and stimulate the secretions of collagen I and collagen Ⅲ. Epithelial cell injury may be involved in the process of oral submucous fibrosis.

关 键 词：上皮细胞 损伤 成纤维细胞 口腔黏膜下纤维性变 胶原 

分 类 号：R781.5[医药卫生—口腔医学]