基于GBS测序的不同来源菊花种质遗传结构分析  

Genetic Structure Analysis of Chrysanthemum Germplasm from Different Sources Based on GBS Sequencing

作　　者：付曼曼 叶琪明[1] 吴超[1] 董彬[2] 方丽[1] 李小白[1] 丁晓瑜[1] 陈炜 陈天烺 郭方其[1] Fu Manman;Ye Qiming;Wu Chao;Dong Bin;Fang Li;Li Xiaobai;Ding Xiaoyu;Chen Wei;Chen Tianlang;Guo Fangqi(Zhejiang Academy of Agricultural Sciences,Hangzhou,310021;College of Landscape Architecture,Zhejiang Agriculture&Forestry University,Hangzhou,311300;Zhejiang Hifun Life Technology Co.,Ltd.,Shaoxing,312000)

机构地区：[1]浙江省农业科学院,杭州310021 [2]浙江农林大学风景园林与建筑学院,杭州311300 [3]浙江海丰生物科技股份有限公司,绍兴312000

出　　处：《分子植物育种》2023年第1期67-79,共13页Molecular Plant Breeding

基　　金：浙江省农业新品种重大专项花卉育种专项(2021C02071-6);浙江省重点研发计划项目(2019C02025)共同资助

摘　　要：菊花(Chrysanthemum morflorium)是中国十大传统名花,也是世界四大切花之一,观赏和经济价值极高。本研究基于单核苷酸多态性(single nucleotidepolymorphism,SNP)检测,对菊花种质资源进行基因分型测序(genotyping-by-sequencing,GBS)来研究菊花的群体遗传结构和亲缘关系。以分别来自中国、日本和荷兰的136份菊花为材料,通过DNA提取、文库构建、测序、酶切后比对到参考基因组,然后进行SNP检测,对进化树、主成分和群体遗传结构进行分析。本研究共得到有效数据183.877Gb,每个样本所得tag数均大于294000;有效测序数据的reads数比对到参考基因组上的reads数的比对率在82.95%~94.61%之间,每个样本的平均测序深度在9.68~15.11之间。进化树构建结果发现136份菊花可被分为2个类群,不同来源的菊花主要聚集在不同的类群上,主成分分析和群体遗传结构结果与此结果一致。通过本研究可以明确不同来源的菊花品种的遗传背景和亲缘关系,对菊花优良种质的挖掘和育种效率的提高具有重要意义。Chrysanthemum morflorium is the top ten traditional flowers in China,and one of the four most famous cut flowers in world,with high ornamental and economic values.In this study,the population genetic structure of chrysanthemum was investigated by genotyping-by-sequencing(GBS)of chrysanthemum germplasm resources based on single nucleotide polymorphism(SNP)detection.A total of 136 chrysanthemums from China,Japan and the Netherlands were used for DNA extraction,library construction,sequencing,enzymatic digestion and then compared to the reference genome,followed by SNP detection and then evolutionary tree,principal components and population genetic structure were analyzed.A total of 183.877 Gb of valid data were obtained,and the number of tags obtained for each sample was greater than 294000;the number of reads from the valid sequencing data were matched to the reference genome in the range of 82.95%~94.61%,and the average sequencing depth of eachsample was in the range of 9.68~15.11. The evolutionary tree revealed that 136 chrysanthemums could be classifiedinto two groups, and chrysanthemums from different sources were mainly clustered on different groups. Consistently,the results of principal component analysis and population genetic structure showed the same trend. Thisstudy can clarify the genetic background of chrysanthemum varieties from different sources, which is important forexcavating excellent germplasm and improving breeding efficiency of chrysanthemum.

关 键 词：菊花 GBS SNP 群体遗传结构 系统进化 

分 类 号：S682.11[农业科学—观赏园艺]