桃PpHDZ1转录因子的克隆及其对冷胁迫的响应  

Cloning of PpHDZ1 Transcription Factor and Its Response to Cold Stress in Peach

作　　者：李清 肖翔 王逸 张琰 陈伟[2] 曹士锋 杨震峰[2] 施丽愉[2] LiQing;Xiao Xiang;WangYi;Zhang Yan;ChenWei;Cao Shifeng;Yang Zhenfeng;Shi Liyu(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306;College of Biological and Environmental Sciences,Zhe-jiangWanli University,Ningbo 315100)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]浙江万里学院生物与环境学院,宁波315100

出　　处：《分子植物育种》2023年第1期80-87,共8页Molecular Plant Breeding

基　　金：浙江省重点研发计划项目(2019C2079)资助。

摘　　要：HD-Zip是植物特异转录因子,在响应冷胁迫过程中起着重要的调控作用。为了解HD-ZipⅠ亚家族转录因子在桃果实采后抗冷害过程中的分子调控机理,本研究从“湖景”水蜜桃叶片中克隆获得了一个编码HD-ZipⅠ亚家族转录因子的基因PpHDZ1,并对该基因的功能进行初步分析。结果表明,PpHDZ1的开放阅读框长度为840 bp,编码氨基酸280个。PpHDZ1蛋白不稳定,呈酸性,具有较强的脂溶性和亲水性,主要由α-螺旋和无规则卷曲组成,无跨膜结构,没有信号肽,可能定位于细胞核。PpHDZ1含有两个高度保守的同源结构域(HD)和亮氨酸拉链结构域(LZ),与扁桃仁、日本杏的同源基因蛋白相似性较高,在系统进化树中与拟南芥HD-ZipⅠ亚家族聚类于同一分支。实时荧光定量PCR (RT-qPCR)结果显示,在不同桃组织中,PpHDZ1仅在硬核期果实中的表达量最高。在桃果实采后低温贮藏期间,0℃、4℃和8℃都显著诱导了PpHDZ1基因的上调表达,其表达强度与桃果实的冷害程度相一致。本研究为揭示HD-Zip转录因子在桃果实抗冷害过程中的分子调控机制提供了理论基础。HD-Zip is a unique transcription factor in plants, which plays an important role in the regulation of cold stress-responsive. In order to understand the mol ecular mechanism of HD-Zip Ⅰ subfamily transcription factors regulating peach fruit resistance to chilling injury, one gene encoding HD-Zip Ⅰ subfamily transcription factor, PpHDZ1, was cloned from ’Hu Jing’ peach leaves and its function was preliminarily analyzed. The results showed that the open reading frame of PpHDZ1 was 840 bp, which encoded 280 amino acids. PpHDZ1 was unstable and acidic, with strong lipid solubility and hydrophilicity. It was mainly composed of alpha helix and random coil structure, and there was no transmemmembrane structure and signal peptide. Subcellular localization prediction showed that PpHDZ1 was localized in the nucleus. PpHDZ1 protein contained two highly conserved domains, HD and LZ, and shared high similarity with homologous protein in Prunus dulcis and Prunus mume. In the phylogenetic tree, Pp HDZ1 was clustered in the same branch with the Arabidopsis HD-Zip Ⅰsubfamily. The RT-qPCR results showed that the expression of PpHDZ1 was the highest only in the hard-core stage fruits among different peach tissues. During the cold storage of peach after harvest, the expression of PpHDZ1 gene was significantly up-regulated at 0 ℃, 4 ℃ and 8 ℃, and its expression level was consistent with the degree of chilling injury of peach fruit. This study laid a theoretical foundation for revealing the molecular mechanism of HD-Zip transcription factor regulating fruit resistance to chilling injury.

关 键 词：桃 HD-ZIP 冷胁迫 表达分析 

分 类 号：S662.1[农业科学—果树学]