白芨转录因子基因BsWRKY14的克隆与分子特性分析  

Molecular Cloning and Characterization of a Transcription Factor Gene BsWRKY14inBletilla striata

作　　者：王双双 窦全丽[1] 赵寅森 曾钢 刘婷 张柏林[1] 彭宇龙 Wang Shuangshuang;Dou Quanli;Zhao Yinsen;Zeng Gang;Liu Ting;Zhang Bolin;Peng Yulong(College of Biological and Agricultural Science and Technology,Zunyi Normal University,Zunyi,563006)

机构地区：[1]遵义师范学院生物与农业科技学院,遵义563006

出　　处：《分子植物育种》2023年第1期123-129,共7页Molecular Plant Breeding

基　　金：遵义师范学院赤水河流域资源保护与开发研究院开放基金项目(遵师CSHKF[2019]06号);遵义市科技局项目(遵市科合计[2018]11号);遵义市创新人才团队培养项目(遵市科人才[2018]1号;遵市科合HZ字[2020]14号)共同资助

摘　　要：WRKY转录因子在调节植物生长发育、抵抗生物和非生物胁迫响应中发挥重要作用,分析WRKY家族成员的基因序列信息,为进一步研究基因功能提供前期研究基础。基于白芨转录组数据,对Bs WRKY14基因进行克隆;利用生物信息学方法对BsWRKY14蛋白的理化特征、保守结构域和亚细胞定位等特性进行分析,用DNAMAN和MEGA 6.0进行氨基酸多序列比对和进化关系分析。结果表明Bs WRKY14基因包含1个1 179 bp的开放阅读框,编码392个氨基酸,预测是定位在细胞核中的不稳定亲水性蛋白,无跨膜结构域,BsWRKY14蛋白包含一个WRKY基因保守结构域和一个C2H2锌指结构域,属于WRKY基因家族的第Ⅱ类成员;Bs WRKY14与铁皮石斛WRKY蛋白亲缘关系最近。白芨Bs WRKY14基因的克隆与分子特性分析为深入研究BsWRKY14基因在调节白芨生长发育和逆境胁迫响应中的作用提供数据支持。WRKY transcription factors play an important role in regulating plant growth and development, and the response to biotic and abiotic stress responses. The study of the sequence information of WRKY family members will provide a preliminary research basis for further research on gene function. Based on the Bletilla striata transcriptome data, identification and sequence analysis on Bs WRKY14 gene were conducted by using bioinformatics methods, and physicochemical properties, structural domains of the protein and subcellular localization of the deduced Bs WRKY14 were analyzed, amino acid multiple sequence alignment and phylogenetic tree analysis of BsWRKY14 were constructed by DNAMAN and MEGA 6.0. The results showed that the cloned BsWRKY14 gene contained a 1 179 bp open reading frame and encoding 392 amino acids. The BsWRKY14 was a unstable hydrophilic protein located in the nucleus and did not contain a transmembrane region. The BsWRKY14 protein contains a WRKY conserved structure and a C2H2zinc finger domain, which belongs to the class Ⅱ member of WRKY gene family. Bs WRKY14 had the closest relationship with the WRKY protein of Dendrobium officinale.Molecular cloning and characteristics analysis of BsWRKY14 will be useful for further determination of the gene in regulating the growth and development and stress resistance of B. striata.

关 键 词：白芨 WRKY 转录因子 克隆 蛋白结构 

分 类 号：S567.239[农业科学—中草药栽培]