异鼠李素对口腔鳞癌细胞增殖、迁移和凋亡的影响及其机制研究  

Effects and mechanism of isorhamnetin on proliferation,migration and apoptosis of oral squamous cell carcinoma cells

作　　者：冯冰华 粟小平 李翠萍 韦珍夏 吕薇 黄旋平[1] Feng Binghua;Su Xiaoping;Li Cuiping;Wei Zhenxia;Lyu Wei;Huang Xuanping(Department of Oral and Maxillofacial Surgery,College&Hospital of Stomatology,Guangxi Medical University,Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction,Guangxi Clinical Research Center for Craniofacial Deformity,Nanning 530021,China)

机构地区：[1]广西医科大学口腔医学院/附属口腔医院颌面外科,广西口腔颌面修复与重建研究自治区级重点实验室,广西颅颌面畸形临床医学研究中心,南宁530021

出　　处：《广西医科大学学报》2022年第12期1951-1957,共7页Journal of Guangxi Medical University

基　　金：广西科技基地和人才专项(No.2021AC18031);广西医疗卫生适宜技术开发与推广应用项目(No.S2021085);南宁市青秀区科技计划项目(No.2021004)。

摘　　要：目的:探讨异鼠李素对口腔鳞癌细胞(SCC-9、SAS)增殖、迁移和凋亡的影响及其机制。方法:体外培养SCC-9、SAS细胞,加入不同浓度的异鼠李素,CCK-8法检测细胞活力。将SCC-9、SAS两种细胞分别分为3组:对照组(不予处理)、低剂量组和高剂量组。用平板克隆实验检测细胞集落形成情况,划痕实验和Transwell迁移实验检测细胞水平及垂直迁移情况,流式细胞术检测细胞凋亡,Western blotting法检测PI3K/AKT/mTOR信号通路相关蛋白表达。结果:随着异鼠李素浓度增加,SCC-9、SAS细胞增殖抑制率升高(P<0.05)。与对照组相比,两种细胞的低剂量组和高剂量组克隆形成率和水平迁移率降低,垂直迁移细胞数减少,两种细胞的高剂量组细胞凋亡率升高(均P<0.05)。SCC-9、SAS细胞高剂量组PI3K110、PI3K85、AKT、p-AKT、mTOR、p-mTOR、S6、p-S6蛋白表达水平均低于对照组(P<0.05)。结论:异鼠李素可能通过抑制PI3K/AKT/mTOR信号通路抑制口腔鳞癌细胞的增殖、迁移,并促进细胞凋亡。Objective:To investigate the effects and mechanism of isorhamnetin on proliferation,migration and apoptosis of oral squamous cell carcinoma(SCC-9,SAS)cells.Methods:SCC-9 and SAS cells were cultured in vitro,and different concentrations of isorhamnetin were added to detect the cell viability by CCK-8 method.SCC-9 and SAS cells were divided into three groups:control group(no treatment),low-dose group and high-dose group.Cell colony formation was detected by plate cloning assay.The horizontal and vertical migration of cells were detected by scratch and Transwell migration assay.Cell apoptosis was detected by flow cytometry,and protein expressions related to PI3K/AKT/mTOR signaling pathway were detected by Western blotting.Results:With the increase of isorhamnetin concentration,the proliferation inhibition rate of SCC-9 and SAS cells increased(P<0.05).Compared with the control group,the clone formation rate and horizontal migration rate decreased in the low-dose and high-dose groups of the two kinds of cells,the number of vertical migration cells decreased,and the apoptosis rate increased in the high-dose group of the two kinds of cells(all P<0.05).The protein expressions of PI3K110,PI3K85,AKT,p-AKT,mTOR,p-mTOR,S6 and p-S6 in SCC-9 and SAS cell highdose groups were lower than those in the control group(P<0.05).Conclusion:Isorhamnetin may inhibit the proliferation as well as the migration and promote the apoptosis of SCC-9 and SAS cells by inhibiting PI3K/AKT/mTOR signaling pathway.

关 键 词：异鼠李素 口腔鳞状细胞癌 增殖 迁移 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]