不同小麦品种花青素含量多样性及其合成基因表达分析  

Analysis of Anthocyanin Content and Synthesis Related Gene Expression in Different Wheat Cultivars

作　　者：苏培森 隋超 颜君[2] 王晨 韩磊 王舒涵 刘晓倩 郭尚敬[1] Su Peisen;Sui Chao;Yan Jun;Wang Chen;Han Lei;Wang Shuhan;Liu Xiaoqian;Guo Shanjing(College of Agronomy and Agricultural Engineering,Liaocheng University,Liaocheng 252000,China;College of Information Science and Engineering,Shandong Agricultural University,Taian 271018,China)

机构地区：[1]聊城大学农学与农业工程学院,山东聊城252000 [2]山东农业大学信息与工程学院,山东泰安271018

出　　处：《山东农业科学》2022年第12期9-17,共9页Shandong Agricultural Sciences

基　　金：聊城大学博士科研启动基金项目(318052018);山东农业大学作物生物学国家重点实验室开放课题(2021KF03);山东省高等学校科技计划项目(J18KA117);泰安市2019年科学技术发展计划(引导计划)项目(2019NS080)。

摘　　要：花青素是植物中广泛存在的一类重要的类黄酮化合物,在植物生长发育和人类身心健康尤其心血管疾病预防方面具有重要价值。小麦作为世界上三大粮食作物之一,其制品是人类膳食的主要来源。筛选富含花青素的小麦种质资源,挖掘其合成调控基因,对培育功能性小麦品种、提高国民健康具有重要意义。本研究以58个不同地区栽培小麦品种为材料,测定其花青素含量,结果显示,不同小麦品种花青素含量差异明显,其中,红麦、紫优6号、Arz、生选6号、宛麦20、紫优5号的花青素含量较高,浙徐福75-3、泡子麦、苏麦3号、淮麦等的花青素含量较低。进一步通过实时荧光定量PCR检测花青素合成通路基因的表达量,结果表明,10个基因在生选6号中的表达量明显高于其他品种,而苏麦3号中TaMYB-7D1、TaMYC-2A1、TaCHI-5D、TaPAL-6D1-T2、TaDFR-3B-T1、TaUFGT、TaCHS和TaF3′5′H基因的表达量最低,且不同小麦品种花青素合成相关基因的表达模式存在差异。本研究结果可为功能性小麦品种培育提供基因资源和种质资源储备。Anthocyanins are a class of important flavonoid compounds in the plant, which have important value in plant growth and development, human physical and mental health especially in the prevention of cardiovascular diseases. As one of the three major cereals in the world, wheat products are the main sources of human diet. Screening anthocyanin-rich wheat germplasm resources and identifying its synthetic regulatory genes have significant impacts on breeding functional wheat cultivars and improving national health. In this study, 58 wheat cultivars were used to determine the anthocyanin content. The results showed that the anthocyanin contents in different wheat cultivars were obviously different. The anthocyanin content in Red Wheat, Ziyou 6, Arz, Shengxuan 6, Wanmai 20 and Ziyou 5 were higher, and that in Zhexufu 75-3, Paozimai, Sumai 3,Huaimai and so on were lower. Furtherly, the expression level of anthocyanin synthesis pathway genes were detected by real-time quantitative PCR. The results showed that the expression level of 10 genes in Shengxuan 6 were significantly higher than that in other varieties, while the expression level of TaMYB-7D1, TaMYC-2A1, TaCHI-5D, TaPAL-6D1-T2, TaDFR-3B-T1, TaUFGT, TaCHS and TaF3′5′H genes in Sumai 3 were the lowest. The expression pattern of these genes were different in different wheat cultivars. These results could provide gene and germplasm resources for functional wheat breeding.

关 键 词：小麦 花青素含量 合成通路基因 表达分析 

分 类 号：S512.1[农业科学—作物学] Q786[生物学—分子生物学]