巨藻Lessonia nigrescens岩藻多糖对烟草幼苗低温胁迫的缓解效应  

Alleviating Effects of Fucoidan from Lessonia nigrescens on Low Temperature Stress to Tobacco Seedlings

作　　者：邹平[1] 杨霞 云龙 龚鹏飞 丁朋辉 周家新 杜秀春 杨伟 徐海波 李义强 曹亚凡 Zou Ping;Yang Xia;Yun Long;Gong Pengfei;Ding Penghui;Zhou Jiaxin;Du Xiuchun;Yang Wei;Xu Haibo;Li Yiqiang;Cao Yafan(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Yan'an Branch of Shaanxi Provincial Tobacco Company,Yan'an 716000,China;Huahuan International Tobacco Co.,Ltd.,Chuzhou 233121,China;Shandong Qingdao Tobacco Co.,Ltd.,Qingdao 266000,China;Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China)

机构地区：[1]中国农业科学院烟草研究所,山东青岛266101 [2]陕西省烟草公司延安市公司,陕西延安716000 [3]华环国际烟草有限公司,安徽滁州233121 [4]山东青岛烟草有限公司,山东青岛266000 [5]上海烟草集团有限责任公司,上海200082

出　　处：《山东农业科学》2022年第12期97-103,共7页Shandong Agricultural Sciences

基　　金：中国农业科学院科技创新工程项目(ASTIP-TRIC06);上海烟草集团科技项目(2021-40800);山东青岛烟草有限公司科技项目(2021-4)。

摘　　要：针对生产中烟草幼苗易遭受低温影响的问题,以雪茄烟H382为试材,设置4℃低温胁迫及喷施不同浓度(100、500、1 000 mg/L)岩藻多糖溶液处理,研究岩藻多糖对低温胁迫下烟草幼苗生理指标的缓解效应。结果表明,低温能够对烟草幼苗造成渗透胁迫和氧化伤害,严重影响植株生长发育。不同浓度的岩藻多糖处理可以明显降低烟草幼苗叶片中超氧阴离子(O_(2)^(-))产生速率、过氧化氢(H_(2)O_(2))和丙二醛(MDA)含量,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化酶活力;同时,还可以增加烟叶中脯氨酸、可溶性糖及可溶性蛋白等渗透调节物质的积累量。说明岩藻多糖能够缓解低温造成的氧化损伤,并增强烟草幼苗渗透调节水平,从而提高其耐低温能力,以500 mg/L岩藻多糖效果最优。本研究可为降低低温胁迫对烟草苗期的不良影响提供参考依据,同时也可以细化海藻多糖的农业应用。In view of the actual production problem that tobacco seedlings are easily affected by low temperature, the low temperature sensitive variety Cigar H382 was used as the test material, and the alleviating effects of different concentrations(100,500,1 000 mg/L) of fucoidan from Lessonia nigrescens on physiological indicators of tobacco seedlings under 4℃ low temperature stress was studied. The results showed that low temperature caused osmotic stress and oxidative damage to tobacco seedlings, which seriously affected the growth and development of tobacco seedlings. Different concentrations of fucoidan obviously reduced the superoxide anion (O_(2)^(-)) production rate, hydrogen peroxide(H_(2)O_(2)) and malondialdehyde(MDA) contents, and enhanced the activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT). Moreover, they also increased the contents of osmotic adjustment substances such as proline, soluble sugar and soluble protein in the leaves of tobacco seedlings. It showed that fucoidan could reduce the membrane lipid peroxidation, improve the osmotic adjustment ability, thereby enhanced the tolerance to low temperature stress. The effect of 500 mg/L fucoidan was the best. These results could provide bases for alleviating the impact of low temperature on tobacco seedlings, and also could refine the agricultural application of seaweed polysaccharides.

关 键 词：岩藻多糖 低温胁迫 烟草幼苗 渗透调节 抗氧化活性 

分 类 号：S572[农业科学—烟草工业]