花生果腐病致病真菌的分离及抗性种质资源鉴定  

Isolation of Pod Rot Pathogenic Fungi and Identification of Resistant Peanut Germplasms

作　　者：朱立飞 张初署[1] 唐月异[1] 韦潇 王冕[1] 于强 宋福荣 张建成[1] Zhu Lifei;Zhang Chushu;Tang Yueyi;Wei Xiao;Wang Mian;Yu Qiang;Song Furong;Zhang Jiancheng(Shandong Peanut Research Institute/Key Laboratory of Biology,Genetics and Breeding of Peanut,Ministry of Agriculture and Rural Affairs,Qingdao 266100,China;Liaoning Technical University,Fuxin 123000,China;Qingdao Tianxiang Food Group Co.,Ltd.,Qingdao 266100,China)

机构地区：[1]山东省花生研究所/农业农村部花生生物学与遗传育种重点实验室,山东青岛266100 [2]辽宁工程技术大学,辽宁阜新123000 [3]青岛天祥食品集团有限公司,山东青岛266100

出　　处：《山东农业科学》2022年第12期116-122,共7页Shandong Agricultural Sciences

基　　金：山东省自然科学基金面上项目(ZR2020MC103,ZR2021MC040);山东省农业科学院农业科技创新工程项目(CXGC2022B06,CXGC2022F33);新疆维吾尔自治区重大科技专项(2022A02008-4)。

摘　　要：花生果腐病严重危害花生的产量和品质。为研究花生果腐病致病真菌及其致病性,本试验以花育917为材料,从发生花生果腐病的籽仁中分离鉴定出一株真菌(PPRF-05),结合形态学观察及ITS序列分析对该菌种进行分类鉴定及致病性分析,同时对实验室保存的花生资源进行抗病性鉴定。结果显示:PPRF-05为尖孢镰刀菌(Fusarium oxysporum),能侵染花生各组织以及花生种子的任一发育阶段;通过离体接种筛选得到不同抗性的种质资源,建立了一套离体接种快速筛选抗病材料的方法。Peanut pod rot seriously harms the yield and quality of peanut. In order to study the pathogenic fungi of peanut pod rot and its pathogenicity, a fungus strain(PPRF-05) was isolated and identified from peanut seed kernel(Huayu 917). Combined with morphological observation and ITS sequence analysis, the classification and pathogenicity analysis of the strain was carried out. At the same time, the peanut resources preserved in our laboratory were identified for the disease resistance. The results showed that PPRF-05 belonged to Fusarium oxysporum, and could infect all peanut tissues and any developmental stage of peanut seeds. By inoculating fresh peanut seeds and cotyledons of different varieties in vitro, the germplasm resources with different resistance were selected, and a rapid screening method for resistant materials in vitro was established.

关 键 词：花生果腐病 尖孢镰刀菌 抗病鉴定 种质资源筛选 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]