黄芪多糖对镉染毒大鼠肝肾损伤改善和肠道菌群结构调节的作用  

Effects of astragalus polysaccharides on improvement of liver and kidney injury and regulation of intestinal flora structure in cadmium-exposed rats

作　　者：邢喜平 何玉林[2] 薛娜[3] 颜春鲁[3] 伍志伟 XING Xi-ping;HE Yu-lin;XUE Na;YAN Chun-lu;WU Zhi-wei(Affiliated Hospital of Gansu University of Chinese Medicine,Lanzhou 730000,China;College of Basic Medical Science Guilin Medical University,Guilin 541199,China;Gansu University of Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学附属医院,甘肃兰州730000 [2]桂林医学院基础医学院,广西桂林541199 [3]甘肃中医药大学,甘肃兰州730000

出　　处：《中国药理学通报》2023年第2期332-339,共8页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 32460140);甘肃省高等学校创新基金项目(No 2021A-082)。

摘　　要：目的探讨黄芪多糖对镉染毒大鼠肝肾损伤改善和肠道菌群结构调节的作用。方法以CdCl 2腹腔注射建立镉染毒大鼠模型,经黄芪多糖持续灌胃处理5周后,采集尿液、肝脏、肾脏和粪便,应用原子吸收光谱法检测尿液、肝脏和肾脏中的镉残留,HE染色观察肝肾病理学变化,Illumina PE250测序和生物信息学软件分析肠道菌群结构。结果注射CdCl 2后,尿液、肝脏和肾脏中镉蓄积明显增加,部分肝肾细胞出现肿胀、坏死、炎性细胞浸润等病理损伤,Chao、ace和shannon指数明显减小,simpson明显增大,OTU减少,Ruminococcus、Bacteroides、Flavonifractor、Roseburia和Elusimicrobium丰度明显下降,Lactobacillus、Lachnospiracea_incertae_sedis、Parasutterella、Clostridium XlVb、Clostridium XI、Intestinimonas和Fusobacterium丰度明显上升,与正常组相比,差异均有统计学意义(P<0.05或P<0.01);黄芪多糖处理后,尿液、肝肾中镉蓄积明显降低,肝肾细胞损伤减轻、炎性细胞浸润减少,Chao、ace和Shannon指数明显上升,simpson明显下降,OTU增加,Prevotella、Bacteroides、Parasutterella、Elusimicrobium和Barnesiella丰度明显上升,Ruminococcus、Oscillibacter、Flavonifractor、Clostridium XlVa、Roseburia、Lactobacillus、Ruminococcus2、Lachnospiracea_incertae_sedis、Clostridium IV、Clostridium XlVb、Clostridium XI和Intestinimonas丰度明显下降,与单纯镉染毒组比较,差异有统计意义(P<0.05或P<0.01)。结论黄芪多糖可能通过减少镉染毒大鼠体内镉蓄积和调节肠道菌群结构,从而改善肝肾组织损伤。Aim To investigate the effects of astragalus polysaccharides on the improvement of liver and kidney injury and the regulation of intestinal flora structure in cadmium exposed rats.Methods Rats exposed to cadmium were established by intraperitoneal injection of CdCl 2.After continuous intragastric administration of astragalus polysaccharides for five weeks,urine,liver,kidney and feces were collected.The cadmium residues in urine,liver and kidney were detected by Graphite Furnace Atomic absorption spectrometry,the pathological changes of liver and kidney were observed by HE staining,and Illumina PE250 sequencing and bioinformatics software were used to analyze the structure of intestinal flora.Results After intraperitoneal injection of CdCl 2,the accumulation of cadmium in urine,liver and kidney increased significantly,some liver and kidney cells showed pathological damage such as swelling,necrosis and inflammatory cell infiltration.Chao,ace and shannon indexes decreased significantly,while simpson index increased significantly.The number of OTU decreased.And the abundance of Ruminococcus,Bacteroides,Flavonifractor,Roseburia and Elusmicrobium decreased significantly,but Lactobacillus,that of Lachnospiracea_incertae_sedis,Parasutterella,Clostridium XlVb,Clostridium XI,Integinimonas and Fusobacterium increased significantly.Compared with the normal control group,the differences was statistically significant(P<0.05 or P<0.01).After intragastric administration of astragalus polysaccharides,cadmium accumulation in urine,liver and kidney decreased significantly,liver and kidney cell damage alleviated,and inflammatory cell infiltration reduced.Chao,ace and shannon index increased markedly,and simpson index decreased significantly.OTU number increased.And the bundance of Prevotella,Bacteroides,Parasutterella,Elismicrobium and Barnesiella raised significantly,that of Ruminococcus,Oscillibacter,Flavonifractor,Clostridium XlVa,Roseburia,Lactobacillus,Ruminococcus2,Lachnospiracea_incertae_sedis,Clostridium IV,Clostridium XlVb,

关 键 词：黄芪多糖 CdCl 2 WISTAR大鼠 肠道菌群 高通量测序 肝肾损伤 

分 类 号：R-332[医药卫生] R284.1R322.45R322.47R916.3