一株产生生物表面活性剂的海洋细菌培养条件优化与产物特性研究  

Optimization of culture conditions of a biosurfactant-producing marine bacterium and characteristics research of its product

作　　者：吴亮[1,2] 王新新 吴岢芯 梁生康 彭昌盛[1] 李泽利[5] WU Liang;WANG Xin-xin;WU Ke-xin;LIANG Sheng-kang;PENG Chang-sheng;LI Ze-li(College of Environmental Science and Engineering,Ocean University of China,Qingdao 266100,China;CNOOC EnerTech-Beijing Safety and Environmental Protection Engineering Technology Research Institute,Beijing 100123,China;College of Environment,Zhejiang University of Technology,Hangzhou 310014,China;College of Chemistry and Chemical Engineering,Ocean University of China,Qingdao 266100,China;Tianjin Eco-Environmental Monitoring Center,Tianjin 300191,China)

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]中海油能源发展股份有限公司北京安全环保工程技术研究院,北京100123 [3]浙江工业大学环境学院,杭州310014 [4]中国海洋大学化学化工学院,山东青岛266100 [5]天津市生态环境监测中心,天津300191

出　　处：《海洋环境科学》2022年第6期897-903,共7页Marine Environmental Science

基　　金：山东省重大科技创新工程(2021CXGC010705)。

摘　　要：微生物代谢产生的生物表面活性剂在海洋环境污染的生物处理方面具有较大应用潜力。从青岛近岸海水中分离到一株生物表面活性剂产生菌株S-22,鉴定为球型节杆菌(Arthrobacter globiformis),通过摇瓶实验对其生长和产生生物表面活性剂的培养条件进行优化,最佳培养条件下6 h表面张力可降低至30.5 mN/m。该菌株产生生物表面活性剂的速度快,有利于大规模工业生产。综合薄层层析分析、傅立叶变换红外光谱分析和GC-MS分析,结果表明该生物表面活性剂是一种由海藻糖和两种脂肪酸(十六烷酸和9-双键十八烷酸)组成的海藻糖脂。该海藻糖脂表面活性高,临界胶束浓度为48.5 mg/L,具有良好的乳化能力,且耐盐和耐热能力较强,具有良好的应用前景。Microbial metabolism can produce biosurfactant,which have potential for biological treatment of marine environmental pollution.An Arthrobacter globiformis strain S-22 was isolated from the offshore seawater of Qingdao.The optimum conditions for its growth and production of biosurfactant were determined by shaking flask experiment.The surface tension decreased to 30.5 mN/m at optimum condition in 6 h.The synthesis of biosurfactant was fast in strain S-22,which was helpful for large scale industrial production.The results of TLC,FT-IR and GC-MS analyses showed that the biosurfactant was a glycolipid composed of trehalose and two fatty acids(hexadecanoic acid and 9-octadecanoic acid).The trehalose lipid,whose critical micelle concentration was as low as 48.5 mg/L,had a fine emulsification capacity with high surface-activity and strong endurance to heat and salts.

关 键 词：海洋细菌 特性 生物表面活性剂 海藻糖脂 培养 

分 类 号：X172[环境科学与工程—环境科学]