紫斑牡丹花粉破壁工艺优化及其金属元素测定  

Optimization of Wall-breaking Technology and Determination of Metal Elements in Paeonia rockii Pollen

作　　者：王新娣[1] 石晓峰[1] 李运 沈薇[1] 刘东彦[1] 范彬[1] 马趣环[1] WANG Xin-di;SHI Xiao-feng;LI Yun(Gansu Academy of Medical Science,Lanzhou,Gansu 730050;Lanzhou Institute for Food and Drug Control,Lanzhou,Gansu 730030)

机构地区：[1]甘肃省医学科学研究院,甘肃兰州730050 [2]兰州市食品药品检验检测研究院,甘肃兰州730030

出　　处：《安徽农业科学》2022年第24期167-171,213,共6页Journal of Anhui Agricultural Sciences

基　　金：兰州市科技发展指导性计划项目(2019-ZD-132);甘肃省医学科学研究院中青年科技人才创新工程项目(ykz-2018-2);兰州市人才创新创业项目(2021-RC-112)。

摘　　要：[目的]优化紫斑牡丹花粉温差破壁工艺,并分析破壁前后10种金属元素的含量变化。[方法]根据Box-benhnken试验设计原理,以解冻温度、解冻时间、液料比为响应变量,以破壁率为响应值设计紫斑牡丹花粉温差破壁工艺,并采用电感耦合等离子体质谱法(ICP-MS)对紫斑牡丹花粉破壁前、破壁后10种金属元素的含量变化进行研究。[结果]最优破壁工艺为冷冻温度-150℃、冷冻时间24 h、液料比20∶1(mL∶g)、解冻时间7 h、解冻温度20℃,在此最优条件下得到牡丹花粉的平均破壁率为92.82%,经平行验证试验后,优化得到牡丹花粉温差破壁工艺,合理可行。花粉经冷冻破壁后,固体颗粒中的Mn、Zn、Sr、Fe、K、Mg、Ca等金属元素的含量均有所下降,有害元素Pb、As、Cd的含量很低,均低于检出限。[结论]该研究为紫斑牡丹花粉的深度开发和综合利用提供理论依据。[Objective]To optimize the wall-breaking technology of Paeonia rockii pollen by temperature difference,10 metal elements in Paeonia rockii pollen were determined before and after wall-breaking of pollen.[Method]According to the Box benhnken test design principle,taking thawing temperature,thawing time,liquid-material ratio as response variables,and wall-breaking rate as response values,the temperature difference wall-breaking process of Paeonia rockii pollen was designed,and the content changes of 10 metal elements in the pollen of Paeonia rockii pollen before and after wall-breaking were studied by inductively coupled plasma mass spectrometry(ICP-MS).[Result]The optimal wall-breaking process was freezing temperature-150℃,freezing time 24 hours,liquid-material ratio 20∶1(mL∶g),thawing time 7 hours,and thawing temperature 20℃.Under this optimal condition,the average wall breaking rate of Paeonia rockii pollen was 92.82%;after parallel verification test,the temperature difference wall-breaking process of Paeonia rockii pollen was optimized,which was reasonable and feasible.The content of Mn,Zn,Sr,Fe,K,Mg,Ca and other metal elements in the solid particles decreased after the pollen was broken by freezing,and the content of harmful elements Pb,As,Cd was very low,which was lower than the detection limit.[Conclusion]This study provides a theoretical basis for the further development and comprehensive utilization of the pollen of Paeonia rockii.

关 键 词：紫斑牡丹花粉 破壁 工艺优化 微波消解 电感耦合等离子体质谱法 金属元素 

分 类 号：TS207.3[轻工技术与工程—食品科学]